FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392142889
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1148650.035.8430251
TCGGAAG1290800.031.8385932
CGGAAGA1489300.027.6418763
AGAGCAC3813150.011.3192117
TATGCCG820300.010.72256145-49
AGCACAC4141900.010.4731959
GAAGAGC4214650.010.3599885
GAGCACA4236050.010.3036368
CTCGTAT798650.09.62237140-44
CGTATGC935900.09.39194945-49
TCTCGTA832700.09.2202140-44
ATGCCGT1037700.08.57242245-49
TTACTCG1004550.08.40843630-34
TACTCGA1068100.08.34494935-39
GCCGTCT1068350.08.2101550-54
TGCCGTC1042600.08.10309645-49
ATCTCGT1019750.07.7021540-44
TCGTATG992150.07.516651640-44
CGTCTGA1249000.07.289338615-19
ACACGTC1279450.07.13061810-14
Produced by FastQC (version 0.11.5)