FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431440353
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1398600.045.7784961
TCGGAAG1523200.042.0858232
CGGAAGA1727750.037.0619773
AGAGCAC4265500.015.6238767
AGCACAC4646000.014.3051119
GAGCACA4706050.014.2028258
GAAGAGC4717350.014.1934275
TATGCCG1029750.013.77235145-49
CGTATGC1157050.012.24206445-49
CTCGTAT994450.012.16330340-44
TCTCGTA1032050.011.7159540-44
ATGCCGT1261950.011.36919645-49
GCCGTCT1288000.010.973783550-54
TACTCGA1302250.010.97060135-39
TTACTCG1249150.010.45968930-34
TGCCGTC1259100.010.38048745-49
ATCTCGT1236800.09.90651340-44
CGTCTGA1492150.09.76391915-19
AAGAGCA7212450.09.5767996
ACACGTC1516450.09.57212410-14
Produced by FastQC (version 0.11.5)