FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431440353
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1915450.032.802699
GAGCGTC2216300.028.4578468
ATCGGAA2456450.026.2039811
TCGGAAG2591300.024.829152
AGAGCGT2621600.024.2020537
CGGAAGA2812800.022.891973
AAGAGCG3058900.020.9862376
GAAGAGC5840050.011.5085685
CGCCGTA1178550.011.31204555-59
TCGCCGT1251900.010.58667455-59
GTCGCCG1383450.09.93975355-59
GCCGTAT1428400.09.47661955-59
TGGTCGC1548250.09.03332350-54
CGTATCA1628900.08.38351360-64
GTGGTCG1710600.08.26413750-54
CCGTATC1507700.08.20441555-59
GGTCGCC1568950.08.15543350-54
TCCGATC306300.07.62114334
GCGTCGT1856000.07.434700510-14
GGAAGAG9499900.07.3724924
Produced by FastQC (version 0.11.5)