FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432809675
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2309500.028.0083481
AGCGTCG2400650.027.4114699
GAGCGTC2719950.024.2649848
ATCGGAA2925050.022.9551431
AGAGCGT3058950.021.6961217
TCGGAAG3095850.021.6275672
CGGAAGA3348000.019.9899353
AAGAGCG3582650.018.6498856
CGCCGTA1742350.016.10449255-59
TCGCCGT1831400.015.29848355-59
GTCGCCG2036900.014.08723455-59
CGTATCA2105400.013.42753160-64
GCCGTAT1922350.013.34084355-59
GTGGTCG2292700.012.67782650-54
GCGTCGT2314900.012.32840810-14
TGGTCGC2153400.012.21010950-54
CGTCGTG2349550.012.15954710-14
TCCGATC375250.011.5657033
GTCGTGT2551650.011.25268410-14
GAAGAGC6333250.011.0247945
Produced by FastQC (version 0.11.5)