FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447206911
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC5082010.113638896783507Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2106900.038.8060349
GAGCGTC2427250.033.7680178
ATCGGAA2635600.031.20311
TCGGAAG2797950.029.4288272
AGAGCGT2828100.029.1714867
CGGAAGA3038000.027.120193
AAGAGCG3279650.025.0114026
GAAGAGC6181250.013.7279635
CGCCGTA1359100.012.69834555-59
TCGCCGT1447550.011.89336555-59
GTCGCCG1593200.011.23048855-59
GCCGTAT1597750.010.93423255-59
TGGTCGC1762400.010.17421750-54
CGTATCA1788300.09.80489660-64
TCCGATC364800.09.6779913
CCGTATC1681650.09.50256155-59
GTGGTCG1940800.09.34806650-54
GGTCGCC1781650.09.26106650-54
GGAAGAG10036700.08.8691714
GCGTCGT2032850.08.79639110-14
Produced by FastQC (version 0.11.5)