FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473407186
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG7156830.15117704613803645TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1937700.069.844011
TCGGAAG2091000.064.579232
CGGAAGA2320950.058.123873
AGAGCAC5119950.026.676927
AGCACAC5523700.024.7006879
GAAGAGC5629900.024.4047685
GAGCACA5625450.024.331298
TATGCCG1567800.018.64655945-49
CTCGTAT1484150.017.37415340-44
CGTATGC1695550.017.06207545-49
AAGAGCA8299950.016.8342656
TCTCGTA1536300.016.79476440-44
CGCTCAT1766200.016.17043530-34
TATCTCG1592600.016.0499340-44
ATGCCGT1830650.015.86864845-49
ACCGCTC1861750.015.48615830-34
GCCGTCT1852400.015.28509550-54
GATCGGA793950.015.2303261
GGAAGAG9320300.015.1222324
GTCACCG1973450.014.98383625-29
Produced by FastQC (version 0.11.5)