FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473407186
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC5962660.1259520382523302Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2340100.046.4956639
GAGCGTC2662850.041.0150388
ATCGGAA2935800.039.3979151
TCGGAAG3113050.037.0962642
AGAGCGT3043950.036.023627
CGGAAGA3349850.034.6110043
AAGAGCG3657900.031.5583846
AGCGGCG754650.029.294869
ATCGGGA692550.026.4753671
GAGCGGC1055850.021.21938
GAAGAGC6661950.017.8021495
AGAGCGG1370550.016.520797
ATGAGCG718900.016.2114332
CGCCGTA1577700.014.1018955-59
GAGAGCG1331950.013.1196396
TCGCCGT1647800.013.04771255-59
GCCGTAT1790950.012.56532355-59
GTCGCCG1950700.012.45620355-59
TGAGCGG909500.012.2216693
TGGTCGC2013900.011.66052650-54
Produced by FastQC (version 0.11.5)