FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424548770
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1492650.042.3833471
TCGGAAG1637600.038.5640642
CGGAAGA1838700.034.294433
GATCGGA857900.021.589361
AGAGCAC4345050.014.8820637
TATGCCG1124350.014.37509645-49
AGCACAC4749350.013.7111739
GAAGAGC4832300.013.5080215
GAGCACA4828850.013.4764548
CTCGTAT1087300.013.24514140-44
CGTATGC1252000.012.91986345-49
TCTCGTA1127800.012.80928940-44
CGCTCAT1318650.012.26116430-34
TATCTCG1176350.012.23755940-44
ATGCCGT1361550.011.92498945-49
GCCGTCT1383450.011.53872750-54
ACCGCTC1411900.011.46982130-34
ATCTCGT1327850.010.99067240-44
GTCACCG1507250.010.91857125-29
CCGCTCA1553600.010.49636630-34
Produced by FastQC (version 0.11.5)