FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424548770
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2056750.029.9411989
GAGCGTC2363900.026.2371838
ATCGGAA2589150.024.570361
TCGGAAG2740750.023.1232532
AGAGCGT2717300.022.8642927
CGGAAGA2995600.021.1780433
AAGAGCG3263150.019.2858966
TCCGATC363100.012.3716983
CGCCGTA1354500.011.5271655-59
GAAGAGC5940850.011.1286075
TCGCCGT1442600.010.79908855-59
GCCGTAT1533800.010.26560955-59
GTCGCCG1650200.09.76101155-59
TGGTCGC1746250.09.35431350-54
GATCGGA2001350.09.3507611
CGTATCA1710150.09.23008160-64
CCGATCT493300.08.8712034
GTGGTCG1882850.08.75415250-54
TTCCGAT573900.08.24585152
GCGTCGT1950300.08.15314510-14
Produced by FastQC (version 0.11.5)