FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427155755
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC6525650.15276980173192328Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2156200.055.2242249
GAGCGTC2448800.048.699398
ATCGGAA2698000.047.2056921
TCGGAAG2848900.044.5940632
AGAGCGT2781150.043.008137
CGGAAGA3076000.041.3754653
AAGAGCG3300100.038.553126
AGCGGCG643550.033.9127049
ATCGGGA600550.024.9340341
GAGCGGC890050.024.7810258
GAAGAGC6046200.021.533035
AGAGCGG1192200.018.6516937
CGCCGTA1523900.016.735155-59
TCGCCGT1568550.015.67699755-59
GCCGTAT1726350.014.90785455-59
GTCGCCG1791400.014.88478255-59
TGGTCGC1904600.013.83373150-54
GGAAGAG9689050.013.8265214
CGTATCA1921700.013.31077560-64
GCGTCGT2038100.013.01094510-14
Produced by FastQC (version 0.11.5)