FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377238834
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG32150530.8522592878123465TruSeq Adapter, Index 27 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCCCGTATG4319070.1144916591487503TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA4911600.0120.124851
TCGGAAG5041550.0116.97092
CGGAAGA5249150.0112.147713
AGAGCAC7447550.079.277967
AGCACAC7753200.076.10239
GAAGAGC7838500.075.45895
GAGCACA7838300.075.308568
AAGAGCA9902850.059.85116
GGAAGAG10669250.055.6068954
TATGCCG4593650.025.86467445-49
CTCGTAT4058100.025.19069140-44
TCTCGTA4068300.025.02061840-44
CGTATGC4704700.024.99375345-49
TTACTCG4766300.024.70291930-34
ATGCCGT4775450.024.6820645-49
TGCCGTC4767650.024.47094545-49
TACTCGA4826300.024.3110535-39
GCCGTCT4790300.024.17795650-54
ATCTCGT4247450.023.9495940-44
TCGTATG4234100.023.9186140-44
Produced by FastQC (version 0.11.5)