FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377238834
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC37369290.9906002943482749Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG5605200.0104.527419
GAGCGTC5876450.099.727298
ATCGGAA6107450.097.474271
TCGGAAG6236450.095.239612
AGAGCGT6271700.093.727837
CGGAAGA6456600.091.970033
AAGAGCG6721050.087.9090356
GAAGAGC9070750.065.4358145
GGAAGAG12355800.048.440274
CGCCGTA4701800.024.20317855-59
TCGCCGT4756700.023.81167655-59
GCCGTAT4883200.023.35455355-59
GTCGCCG5119100.022.8982455-59
CCGTATC4948850.022.86985255-59
TGGTCGC5216900.022.67821550-54
GGTCGCC5269500.022.36518150-54
CGTATCA5063700.022.35975560-64
GTGGTCG5440250.021.8121850-54
GCGTCGT5511150.021.33301210-14
CGTCGTG5549600.021.2343210-14
Produced by FastQC (version 0.11.5)