FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446588061
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG20705920.46364696704240826TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3823950.0101.850171
TCGGAAG3982000.097.658882
CGGAAGA4214100.092.140733
AGAGCAC6827950.057.0211077
AGCACAC7217300.053.926689
GAAGAGC7312400.053.4163255
GAGCACA7322100.053.2193578
AAGAGCA9772650.040.1201486
GGAAGAG10820850.036.4062734
TATGCCG3342200.023.32671545-49
CTCGTAT2957600.022.7174140-44
TCTCGTA2978800.022.40970440-44
CGTATGC3477000.022.1759145-49
ATGCCGT3563600.021.63334745-49
TGCCGTC3558650.021.35704645-49
GCCGTCT3585750.021.11883450-54
TCGTATG3165100.020.90146340-44
ATCTCGT3202100.020.86868740-44
CCGGAGA3808100.020.27670130-34
ACTCCGG3861050.020.0395630-34
Produced by FastQC (version 0.11.5)