FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446588061
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC23798800.5329027369587473Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG4730800.081.250699
GAGCGTC5062200.075.95088
ATCGGAA5302650.073.113291
AGAGCGT5458800.070.5443347
TCGGAAG5484300.070.494762
CGGAAGA5727000.067.360573
AAGAGCG6055100.063.6620456
GAAGAGC8850650.043.8934565
GGAAGAG12703850.030.8974294
CGCCGTA3699600.020.20264655-59
TCGCCGT3771550.019.67501655-59
GCCGTAT3863850.019.37836555-59
CCGTATC3962800.018.64425755-59
GTCGCCG4173750.018.53870255-59
TGGTCGC4242050.018.34422350-54
CGTATCA4045050.018.31143460-64
GGTCGCC4381300.017.68541750-54
GTGGTCG4450450.017.56959550-54
GCGTCGT4549700.016.82845310-14
CGTCGTG4577850.016.74302310-14
Produced by FastQC (version 0.11.5)