FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494693495
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC18158940.36707456604012956Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG4406200.065.1590049
GAGCGTC4746750.060.5183148
ATCGGAA5086550.057.4698941
AGAGCGT5201000.055.3562977
TCGGAAG5287950.055.093552
CGGAAGA5551900.052.3300133
AAGAGCG5920500.048.9594736
GAAGAGC8953450.032.7527585
GGAAGAG13088650.022.7290654
CGCCGTA3306800.017.491355-59
TCGCCGT3395150.016.96056455-59
GCCGTAT3470400.016.69806155-59
CCGTATC3579400.015.90081955-59
CGTATCA3671550.015.64350860-64
GTCGCCG3770800.015.58347755-59
TGGTCGC3829200.015.45051450-54
GTGGTCG4033750.014.71266450-54
GGTCGCC4003050.014.639729550-54
GCGTCGT4219800.013.78379710-14
CGTCGTG4253600.013.74958310-14
Produced by FastQC (version 0.11.5)