FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487715582
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG7101950.14561663112908294TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1997550.067.172781
TCGGAAG2136550.062.638832
CGGAAGA2396550.055.7919043
AGAGCAC5208500.026.1379977
AGCACAC5611100.024.2458049
GAGCACA5713200.023.9177638
GAAGAGC5735800.023.836165
TATGCCG1567950.017.43364145-49
AAGAGCA8455350.016.483376
CTCGTAT1456350.016.20684840-44
CGTATGC1723850.015.7762545-49
TCTCGTA1495800.015.77068840-44
ATGCCGT1806350.015.09424145-49
GGAAGAG9373100.014.9471924
TGCCGTC1802200.014.7355945-49
GCCGTCT1844650.014.53047550-54
TCGTATG1685000.013.70989940-44
ATCTCGT1734150.013.707640-44
CCGGAGA2011150.013.4581730-34
ACTCCGG2089700.013.16389330-34
Produced by FastQC (version 0.11.5)