FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487715582
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC8411840.17247429260933478Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2779950.048.1014639
GAGCGTC3132150.042.7894748
ATCGGAA3380000.040.2789881
TCGGAAG3561550.038.102822
AGAGCGT3542850.037.94397
CGGAAGA3854900.035.156073
AAGAGCG4160600.032.5924646
GAAGAGC7236850.019.2127725
CGCCGTA1882100.014.36074755-59
TCGCCGT1975200.013.67211755-59
GCCGTAT2087650.013.0745755-59
GGAAGAG11229850.012.707774
GTCGCCG2219200.012.38119555-59
CCGTATC2204150.012.10199655-59
TGGTCGC2335400.011.89502450-54
CGTATCA2294150.011.812860560-64
GTGGTCG2490950.011.18307450-54
GGTCGCC2447600.011.1554650-54
GCGTCGT2678400.010.21516710-14
CGTCGTG2708250.010.13790610-14
Produced by FastQC (version 0.11.5)