FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481600562
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC11074220.22994616023724657Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3603150.048.7024849
GAGCGTC4029600.043.5905578
ATCGGAA4201600.042.161891
AGAGCGT4328750.040.6707767
TCGGAAG4399700.040.1744962
CGGAAGA4680950.037.6993183
AAGAGCG4975900.035.5306476
GAAGAGC7969850.022.5446245
GGAAGAG11905150.015.3990814
CGCCGTA2430950.015.31497755-59
TCGCCGT2544950.014.59990655-59
GCCGTAT2608300.014.29364455-59
GTCGCCG2846150.013.28753455-59
CGTATCA2802400.013.24169560-64
TGGTCGC2941450.012.979238550-54
CCGTATC2729750.012.69572855-59
GTGGTCG3122400.012.31014550-54
GGTCGCC3062850.011.66607450-54
TCTCGGT3580950.010.88509145-49
TCTTCGC3517550.010.86445730-34
Produced by FastQC (version 0.11.5)