FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391629135
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG8242470.21046621058977136TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2090500.075.6851961
TCGGAAG2207900.071.565942
CGGAAGA2423250.065.193893
AGAGCAC4681650.034.0439877
AGCACAC4958250.032.164459
GAAGAGC5086650.031.5072485
GAGCACA5080300.031.3834848
AAGAGCA7336800.022.0724626
GGAAGAG8047550.020.1725584
TATGCCG1670250.019.49115645-49
CTCGTAT1520450.018.15957640-44
CGTATGC1806400.017.86235445-49
TCTCGTA1542400.017.77141440-44
ATGCCGT1873300.017.27243645-49
TGCCGTC1860050.016.71727645-49
GCCGTCT1900300.016.7134450-54
ATCTCGT1733000.015.90053740-44
TCGTATG1686750.015.74509140-44
CCGGAGA2048500.015.43629430-34
ACTCCGG2112700.015.31312530-34
Produced by FastQC (version 0.11.5)