FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409004829
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG8486550.20749266019057197TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2022000.075.150131
TCGGAAG2152600.070.3559952
CGGAAGA2380400.063.592893
AGAGCAC4761750.032.1454627
AGCACAC5081450.030.1895759
GAGCACA5180300.029.5636228
GAAGAGC5200500.029.5533525
AAGAGCA7479350.020.8366666
TATGCCG1618750.019.04199245-49
GGAAGAG8282100.018.9023744
CTCGTAT1513050.018.16487340-44
TCTCGTA1540250.017.75091240-44
CGTATGC1762500.017.3466345-49
ATGCCGT1840600.016.69879345-49
TGCCGTC1846000.016.3114845-49
GCCGTCT1868100.016.176750-54
ATCTCGT1751900.015.71479540-44
TCGTATG1746050.015.47685640-44
CCGGAGA2001200.015.25151530-34
ACTCCGG2096450.014.90501530-34
Produced by FastQC (version 0.11.5)