FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409004829
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC9449240.2310300351001479Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2957200.051.177099
GAGCGTC3274850.046.286378
ATCGGAA3419300.044.6970521
TCGGAAG3601550.042.464582
AGAGCGT3657800.041.529017
CGGAAGA3862700.039.5504843
AAGAGCG4187400.036.455936
GAAGAGC6698650.023.169335
GGAAGAG10036850.015.7999454
CGCCGTA1994000.015.31644655-59
TCGCCGT2063550.014.72154855-59
GCCGTAT2123550.014.43050955-59
CCGTATC2222200.013.50876355-59
GTCGCCG2332150.013.27774755-59
CGTATCA2300600.013.22721560-64
TGGTCGC2403150.013.04596650-54
GTGGTCG2545000.012.38773650-54
GGTCGCC2533950.012.214107550-54
GTACGTC2626950.011.8351730-34
TACGTCC2696700.011.64782230-34
Produced by FastQC (version 0.11.5)