FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388900909
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG16180650.4160609971729328TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2926950.0103.2421341
TCGGAAG3057400.098.760582
CGGAAGA3237750.093.14113
AGAGCAC5543950.054.682187
AGCACAC5870050.051.6599279
GAGCACA5937700.051.0140158
GAAGAGC5965450.050.96045
AAGAGCA8184350.037.3416566
GGAAGAG8969600.034.191174
TATGCCG2566450.023.8710445-49
CTCGTAT2287900.022.83172840-44
CGTATGC2677450.022.64314745-49
TCTCGTA2323650.022.38998240-44
ATGCCGT2774850.021.90268945-49
TGCCGTC2760450.021.48436545-49
GCCGTCT2781700.021.43317850-54
TCGTATG2436600.020.95757740-44
ATCTCGT2514250.020.7624240-44
ACTCCGG2971600.020.58420230-34
CCGGAGA2930750.020.48969730-34
Produced by FastQC (version 0.11.5)