FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388900909
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC19212300.4940153019801761Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3487800.086.007279
GAGCGTC3772900.079.5600368
ATCGGAA3971200.076.5461041
TCGGAAG4090050.074.1287162
AGAGCGT4150250.072.577777
CGGAAGA4315500.070.189573
AAGAGCG4575900.065.913716
GAAGAGC7071150.043.0273785
GGAAGAG10499750.029.3618354
CGCCGTA2723150.021.8148555-59
TCGCCGT2786650.021.18925355-59
GCCGTAT2925800.020.37622855-59
GTCGCCG2988600.020.29392255-59
TGGTCGC3123300.019.6062150-54
CCGTATC2980700.019.59432455-59
CGTATCA3089550.019.15879860-64
GGTCGCC3150000.019.1450250-54
GTGGTCG3311100.018.57649250-54
GCGTCGT3403750.017.87154610-14
CGTCGTG3444200.017.7096410-14
Produced by FastQC (version 0.11.5)