FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510850347
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC7802470.152734945680677Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3121600.040.285729
GAGCGTC3506950.035.9666828
ATCGGAA3746950.034.247181
TCGGAAG3936850.032.611242
AGAGCGT3950200.031.9950147
CGGAAGA4277850.029.9885883
AAGAGCG4634900.027.52136
GAAGAGC7809450.016.773455
TCCGATC438300.015.1734143
CGCCGTA2010700.012.66615755-59
TCGCCGT2109200.012.0409755-59
GCCGTAT2201350.011.63240455-59
GGAAGAG12049750.011.2340484
CCGATCT586200.011.0846344
GTCGCCG2394400.010.84224555-59
CCGTATC2316050.010.82287855-59
CGTATCA2420600.010.55159360-64
TGGTCGC2494400.010.50775150-54
TTCCGAT696350.010.1473372
GTGGTCG2675700.09.85535250-54
Produced by FastQC (version 0.11.5)