FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400678786
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG12789170.3191875998146805TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2526150.084.609011
TCGGAAG2675850.079.7774052
CGGAAGA2863450.074.623473
AGAGCAC5249300.041.065787
AGCACAC5577200.038.6270339
GAGCACA5680100.037.9677968
GAAGAGC5701700.037.9204675
AAGAGCA7962900.027.3690226
GGAAGAG8872200.024.6701954
TATGCCG2110700.020.92284445-49
CTCGTAT2022250.020.22317140-44
TCTCGTA2053200.019.89855640-44
TCGTATC2251900.019.59627335-39
CGTATGC2239850.019.45944845-49
TATCTCG2104300.019.1797140-44
ATGCCGT2309900.019.0833645-49
TGCCGTC2328900.018.32067545-49
GTCACGA2420900.018.3039525-29
GCCGTCT2365950.018.26111250-54
TCGTATG2175450.018.23251540-44
Produced by FastQC (version 0.11.5)