FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400678786
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC13718390.34237874525256246Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3399700.062.647469
GAGCGTC3704050.057.56058
ATCGGAA3904350.055.7291151
TCGGAAG4086750.053.0357972
AGAGCGT4044700.052.7703067
CGGAAGA4301300.050.3412633
AAGAGCG4596000.047.0023166
GAAGAGC7156600.030.5447565
GGAAGAG10508700.021.0856954
CGCCGTA2515350.016.98214555-59
TCGCCGT2591750.016.42280455-59
GCCGTAT2662950.016.07680555-59
GTCGCCG2862750.015.31130455-59
CCGTATC2745300.015.13191455-59
CGTATCA2812600.015.10255660-64
TGGTCGC2936550.015.04694550-54
GTGGTCG3085000.014.38727450-54
GGTCGCC3052500.014.15714850-54
GCGTCGT3304150.013.19117910-14
CGTCGTG3335300.013.10797310-14
Produced by FastQC (version 0.11.5)