FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439690852
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG12152190.2763803236916105TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2687450.083.221961
TCGGAAG2852950.078.298142
CGGAAGA3070800.072.60273
AGAGCAC5673950.039.6325957
AGCACAC6064650.037.0408869
GAAGAGC6140300.036.6806455
GAGCACA6136050.036.6628
AAGAGCA8616750.026.3666116
GGAAGAG9572300.023.8423374
TATGCCG2214250.020.31833545-49
CTCGTAT2014500.019.41782440-44
TCTCGTA2057750.018.97869740-44
CGTATGC2364650.018.8132545-49
TATCTCG2114050.018.20853240-44
ATGCCGT2461700.018.10689545-49
TGCCGTC2463100.017.80992745-49
TCGTATG2195250.017.5548340-44
GCCGTCT2496400.017.54654950-54
ATCTCGT2294500.017.07036840-44
CGTCTTC2737150.016.29456950-54
Produced by FastQC (version 0.11.5)