FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429202774
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG5261700.12259240430724709TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1707400.053.4011421
TCGGAAG1864800.048.8831832
CGGAAGA2059900.044.3267173
AGAGCAC4631900.020.1254837
AGCACAC4993250.018.7418179
GAAGAGC5105950.018.3861225
GAGCACA5094650.018.3771658
TATGCCG1291300.014.52342245-49
CTCGTAT1256500.013.64241140-44
TCTCGTA1304900.013.15306840-44
CGTATGC1451550.012.82915445-49
AAGAGCA7510700.012.75516
TATCTCG1339900.012.63095140-44
ATGCCGT1518600.012.39160445-49
TCGTATG1394350.012.13874540-44
TGCCGTC1539300.011.98478145-49
GCCGTCT1597750.011.5744750-54
GGAAGAG8474800.011.4824814
ATCTCGT1512850.011.42752140-44
ACACGTC1854750.010.24336910-14
Produced by FastQC (version 0.11.5)