FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429202774
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC5796800.13505970490302563Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2666450.034.616599
GAGCGTC3000050.030.7817468
ATCGGAA3220550.029.7734011
TCGGAAG3412950.027.959522
AGAGCGT3372850.027.4827677
CGGAAGA3625450.026.2198853
AAGAGCG3947950.023.9991976
GAAGAGC6666800.014.6355115
CGCCGTA1726050.010.71827555-59
TCGCCGT1812050.010.15437755-59
GCCGTAT1900300.09.86057955-59
GGAAGAG10297850.09.8018814
GTCGCCG2043950.09.3108655-59
CCGTATC1998600.09.21236755-59
TGGTCGC2127800.09.0720350-54
CGTATCA2062050.09.03380660-64
GGTCGCC2233550.08.59836350-54
GTGGTCG2287700.08.52222650-54
GCGTCGT2557500.07.316130610-14
CGTCGTG2598550.07.24742510-14
Produced by FastQC (version 0.11.5)