FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437265156
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1887100.023.9414549
GAGCGTC2221200.020.4665288
ATCGGAA2436250.018.8188631
AGAGCGT2595200.017.6567337
TCGGAAG2607750.017.598372
CGGAAGA2854700.016.1273333
AAGAGCG3126100.014.7905476
GAAGAGC5927250.08.2084685
CGCCGTA1124050.08.01440955-59
TCGCCGT1198000.07.547471555-59
GCCGTAT1341350.06.966288655-59
GTCGCCG1370300.06.884570655-59
TGGTCGC1509200.06.44365150-54
ACCGCGC1125550.06.3719731
CCGTATC1444800.06.2912655-59
CGTATCA1528800.06.151839760-64
GGTCGCC1580650.06.010514750-54
GTGGTCG1643750.05.932925750-54
GGAAGAG9645450.05.3770364
GCGTCGT1767250.05.243191210-14
Produced by FastQC (version 0.11.5)