FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499196480
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT6698710.1341898484540596TruSeq Adapter, Index 19 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG5560450.11138800497952228TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1588400.068.697631
ATCGGAA2543800.043.074822
TCGGAAG2713950.040.3985023
CGGAAGA2978200.036.783074
TATGCCG2135850.020.1000145-49
CTCGTAT2039300.019.16506440-44
AGAGCAC5921450.018.8656548
TCTCGTA2080650.018.78627240-44
CGTATGC2290500.018.6487345-49
TATCTCG2139200.018.10495440-44
ATGCCGT2404950.017.76907245-49
GAAGAGC6426700.017.5017596
GCCGTCT2430000.017.4069950-54
GAGCACA6475800.017.305439
AGCACAC6316350.017.2060669
ATCTCGT2322350.016.88094340-44
CGTCTGA2696500.015.97798515-19
ACACGTC2729200.015.85032510-14
CGTCTTC2733150.015.69820850-54
CCGTCTT2778200.015.44208850-54
Produced by FastQC (version 0.11.5)