FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499196480
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT6943840.1391003398100884Illumina Single End PCR Primer 1 (96% over 32bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC6466830.12954478364911548Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2984000.036.5770531
AGCGTCG3276900.032.009379
GAGCGTC3642150.029.8357019
ATCGGAA3913600.027.9374732
AGAGCGT4058400.026.8499898
TCGGAAG4087700.026.764693
CGGAAGA4414050.024.854414
AAGAGCG4670500.023.3941487
CGCCGTA2410550.017.2778355-59
TCGCCGT2493450.016.66271655-59
GCCGTAT2626000.015.90772455-59
GTCGCCG2756650.015.45863755-59
TGGTCGC2897700.014.78058950-54
CGTATCA2828850.014.750936560-64
GAAGAGC7836150.014.3750046
GTGGTCG3066050.014.07865450-54
GCGTCGT3157600.013.42653110-14
CGTCGTG3205400.013.23806510-14
TCGTGTA3397400.012.55262610-14
GTCGTGT3433650.012.414618510-14
Produced by FastQC (version 0.11.5)