FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428373542
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG11796460.2753778850328716TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2527000.092.62641
TCGGAAG2678000.087.263442
CGGAAGA2911750.080.091753
AGAGCAC5382950.043.543787
AGCACAC5746150.040.758699
GAAGAGC5842500.040.2962045
GAGCACA5852350.040.0202988
AAGAGCA8248200.028.7304236
GGAAGAG9078550.026.222774
TATGCCG2161150.021.8307345-49
CTCGTAT1913500.020.378840-44
CGTATGC2278950.020.26154545-49
TCTCGTA1940200.019.96523940-44
ATGCCGT2382750.019.67555645-49
GTCACGA2443950.019.45788225-29
TGCCGTC2381250.019.39231745-49
GCCGTCT2402500.018.95145850-54
ACGAGAT2564900.018.47961430-34
TCGTATG2097650.018.16020240-44
ATCTCGT2148500.018.05659540-44
Produced by FastQC (version 0.11.5)