FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428373542
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC14765110.3446783835216415Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3174900.073.502669
GAGCGTC3491050.066.890698
ATCGGAA3672600.064.222941
TCGGAAG3821400.061.6238172
AGAGCGT3840100.060.9016277
CGGAAGA4073000.057.744983
AAGAGCG4328800.054.2348446
GAAGAGC7027600.033.8632135
GGAAGAG10536400.022.922624
CGCCGTA2435750.019.30082155-59
TCGCCGT2518150.018.65371355-59
GCCGTAT2612950.018.05070555-59
GTCGCCG2695450.017.46112855-59
CCGTATC2724900.017.02936455-59
TGGTCGC2813000.016.94225550-54
CGTATCA2787450.016.75858960-64
GGTCGCC2876850.016.33298950-54
GTGGTCG2964600.016.13992150-54
GCGTCGT3108100.015.25376410-14
CGTCGTG3141350.015.13290610-14
Produced by FastQC (version 0.11.5)