FastQCFastQC Report
Fri 2 Sep 2022
EGAF00005002487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270070
Sequences flagged as poor quality0
Sequence length251
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATTCGATGTTGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA14470.5357870181804717No Hit
ATCATTCGATGTTGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA9720.3599066908579257No Hit
GTCATTCGATGTTGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA9160.3391713259525308No Hit
TACATTCGATGTTGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA9080.3362091309660459No Hit
TGCATTCGATGTTGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA8080.29918169363498354No Hit
TCCATTCGATGTTGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA8050.29807087051505166No Hit
CTCATTCGATGTTGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA7750.28696263931573296No Hit
TTCATTCGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG7700.28511126744917986No Hit
TTCATTCGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG6730.2491946532380494No Hit
AACATTCGATGTTGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA6370.23586477579886694No Hit
TACATTCGAGGAATCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC6040.22364572147961637No Hit
TTCATTCGAGGAATCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC6030.22327544710630579No Hit
TTCATTCGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG6030.22327544710630579No Hit
TTCATTCGACGAGGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6010.22253489835968454No Hit
GACATTCGATGTTGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5960.22068352649313142No Hit
AGCATTCGATGTTGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5680.21031584404043396No Hit
CACATTCGATGTTGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5610.2077239234272596No Hit
CTCATTCGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG5480.2029103565742215No Hit
ACCATTCGATGTTGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5470.20254008220091088No Hit
GCCATTCGATGTTGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5440.201429259080979No Hit
TTCATTCGATGTTGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA5370.19883733846780463No Hit
TTCATTCGATGCACCCAGGGTTTTCCCAGTCACGACCTCCCTGTTTATCC5320.1969859666012515No Hit
ATCATTCGAGGAATCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC5260.1947643203613878No Hit
CTCATTCGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG5190.19217239974821343No Hit
AACATTCGAGGAATCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC5150.19069130225497094No Hit
TTCATTCGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG5070.18772910726848596No Hit
TACATTCGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4950.18328581478875847No Hit
ATCATTCGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG4900.18143444292220537No Hit
TTCATTCGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4840.1792127966823416No Hit
TTCATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG4830.17884252230903097No Hit
TTCATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCAGAAGGCAAGA4660.17254785796275038No Hit
TTCATTCGAGGAATCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4640.17180730921612916No Hit
CGCATTCGATGTTGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4590.16995593734957604No Hit
GGCATTCGATGTTGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4390.16255044988336356No Hit
ATCATTCGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG4370.16180990113674232No Hit
TGCATTCGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG4350.1610693523901211No Hit
TCCATTCGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG4340.16069907801681046No Hit
GTCATTCGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG4310.15958825489687858No Hit
TCCATTCGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG4290.15884770615025734No Hit
TGCATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG4270.1581071574036361No Hit
TTCATTCGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG4270.1581071574036361No Hit
CCCATTCGATGTTGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4250.15736660865701485No Hit
CTCATTCGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG4100.1518124930573555No Hit
TACATTCGAGGAATCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4090.15144221868404487No Hit
CTCATTCGAGGAATCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC4050.14996112119080238No Hit
TTCATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4020.1488502980708705No Hit
GTCATTCGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3830.14181508497796866No Hit
CACATTCGAGGAATCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC3820.14144481060465805No Hit
TACATTCGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3750.13885288999148368No Hit
CTCATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3740.13848261561817307No Hit
TTCATTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3740.13848261561817307No Hit
TGCATTCGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG3670.1358906950049987No Hit
AACATTCGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3660.13552042063168807No Hit
TGCATTCGACGAGGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA3650.13515014625837746No Hit
TTCATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA3630.1344095975117562No Hit
GTCATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCAGAAGGCAAGA3610.13366904876513497No Hit
TGCATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCAGAAGGCAAGA3580.1325582256452031No Hit
CTCATTCGATGCACCCAGGGTTTTCCCAGTCACGACCTCCCTGTTTATCC3570.13218795127189248No Hit
GTCATTCGACGAGGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA3560.13181767689858184No Hit
ATCATTCGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3540.1310771281519606No Hit
TTCATTCGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3540.1310771281519606No Hit
ATCATTCGATGTTGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA3520.13033657940533935No Hit
CGCATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3500.1295960306587181No Hit
GTCATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3470.12848520753878623No Hit
CTCATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCAGAAGGCAAGA3470.12848520753878623No Hit
ATCATTCGACGAGGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA3420.12663383567223313No Hit
TACATTCGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG3420.12663383567223313No Hit
AACATTCGAGGAATCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3380.1251527381789906No Hit
GTCATTCGATGTTGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA3370.12478246380568002No Hit
ATCATTCGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG3360.12441218943236938No Hit
TCCATTCGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG3360.12441218943236938No Hit
ATCATTCGATGCACCCAGGGTTTTCCCAGTCACGACCTCCCTGTTTATCC3340.12367164068574814No Hit
GTCATTCGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG3340.12367164068574814No Hit
ATCATTCGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3340.12367164068574814No Hit
ATCATTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3330.12330136631243752No Hit
TACATTCGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3330.12330136631243752No Hit
GTCATTCGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG3310.12256081756581627No Hit
TACATTCGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3270.12107972007257377No Hit
TTCATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG3270.12107972007257377No Hit
TGCATTCGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3260.12070944569926316No Hit
TTCATTCGATGTTGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3250.12033917132595252No Hit
CACATTCGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3240.1199688969526419No Hit
TTCATTCGATGTTGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3230.11959862257933129No Hit
TCCATTCGATGCACCCAGGGTTTTCCCAGTCACGACCTCCCTGTTTATCC3200.11848779945939941No Hit
TTCATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT3190.11811752508608879No Hit
CCCATTCGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3190.11811752508608879No Hit
CTCATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3180.11774725071277818No Hit
GTCATTCGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3170.11737697633946755No Hit
TACATTCGATGTTGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA3160.11700670196615691No Hit
TCCATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3160.11700670196615691No Hit
CCCATTCGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG3140.11626615321953568No Hit
CACATTCGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3130.11589587884622504No Hit
TGCATTCGATGTTGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA3130.11589587884622504No Hit
TACATTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3130.11589587884622504No Hit
TGCATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3130.11589587884622504No Hit
TGCATTCGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3120.11552560447291443No Hit
GTCATTCGATGCACCCAGGGTTTTCCCAGTCACGACCTCCCTGTTTATCC3100.11478505572629319No Hit
CGCATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCAGAAGGCAAGA3090.11441478135298257No Hit
TTCATTCGATGCACCCAGGGTTTTCCCAGTCACGACATCGCTGAAGACAG3090.11441478135298257No Hit
CACATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3080.11404450697967193No Hit
TACATTCGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3080.11404450697967193No Hit
TTCATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC3070.11367423260636132No Hit
TGCATTCGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG3070.11367423260636132No Hit
TTCATTCGATGCACCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA3060.11330395823305069No Hit
TGCATTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3040.11256340948642946No Hit
ACCATTCGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3040.11256340948642946No Hit
TACATTCGACGAGGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA3040.11256340948642946No Hit
GTCATTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3030.11219313511311882No Hit
ATCATTCGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3010.11145258636649757No Hit
GGCATTCGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3010.11145258636649757No Hit
TCCATTCGACGAGGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA3000.11108231199318695No Hit
TCCATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCAGAAGGCAAGA2960.10960121449994446No Hit
TACATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCAGAAGGCAAGA2950.10923094012663384No Hit
TCCATTCGATGTTGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA2950.10923094012663384No Hit
ATCATTCGAGGAATCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT2940.1088606657533232No Hit
TACATTCGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG2940.1088606657533232No Hit
TTCATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA2920.10812011700670196No Hit
CGCATTCGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG2910.10774984263339134No Hit
TCCATTCGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG2890.1070092938867701No Hit
TGCATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG2880.10663901951345947No Hit
CACATTCGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG2880.10663901951345947No Hit
GCCATTCGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG2850.10552819639352762No Hit
CTCATTCGATGTTGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA2820.10441737327359574No Hit
CGCATTCGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG2810.10404709890028511No Hit
TACATTCGATGCACCCAGGGTTTTCCCAGTCACGACCTCCCTGTTTATCC2810.10404709890028511No Hit
TACATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG2810.10404709890028511No Hit
GACATTCGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG2800.10367682452697448No Hit
TTCATTCGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG2790.10330655015366387No Hit
ATCATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG2780.10293627578035325No Hit
GTCATTCGAGGAATCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC2780.10293627578035325No Hit
GGCATTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA2750.10182545266042137No Hit
ACCATTCGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG2740.10145517828711074No Hit
TCCATTCGAAGGAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG2740.10145517828711074No Hit
GACATTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA2730.10108490391380012No Hit
CTCATTCGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA2730.10108490391380012No Hit
TTCATTCGATGCACCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC2720.1007146295404895No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)