FastQCFastQC Report
Thu 1 Sep 2022
EGAF00005002490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26647
Sequences flagged as poor quality0
Sequence length251
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA11034.139302735767628No Hit
TGCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG10503.940406049461478No Hit
AGCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG9823.68521784816302No Hit
TTGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA9633.613915262506098No Hit
AGGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG9203.4525462528614854No Hit
AAGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA8653.246144031223027No Hit
TCGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG8573.216121889893796No Hit
GGCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG8153.0585056479153376No Hit
TCGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA7312.743273163958419No Hit
TGGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA6212.3304687206815022No Hit
GCGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA5562.086538822381506No Hit
CGGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG5001.876383833076894No Hit
CGGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA4851.8200923180845874No Hit
TGGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG4831.8125867827522797No Hit
GGGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG4601.7262731264307427No Hit
CCGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA4491.6849926821030508No Hit
CTGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA4091.5348819754568996No Hit
ACGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA4081.5311292077907457No Hit
GCCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3951.4823432281307465No Hit
CAGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA3411.2796937741584418No Hit
TTCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3371.2646827034938266No Hit
CGGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA3161.185874582504597No Hit
TCGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3121.170863511839982No Hit
GGGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA3111.1671107441738282No Hit
ATGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2951.1070664615153676No Hit
CTCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2660.9982361991969076No Hit
AGGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2660.9982361991969076No Hit
AGGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2470.9269336135399857No Hit
GTCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2460.923180845873832No Hit
AGGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2380.8931587045446017No Hit
CCGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2340.8781476338799865No Hit
TACCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2310.8668893308815252No Hit
GGGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2230.8368671895522949No Hit
GCGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2200.8256088865538334No Hit
TGGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2190.8218561188876797No Hit
TGGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2130.7993395128907569No Hit
TTGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2120.795586745224603No Hit
ACGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2120.795586745224603No Hit
TTGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2100.7880812098922956No Hit
AGGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2090.7843284422261417No Hit
GTGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2020.7580590685630653No Hit
ATCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2000.7505535332307577No Hit
CTGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1960.7355424625661425No Hit
CTGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1950.7317896948999887No Hit
TCCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1900.7130258565692198No Hit
ACGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1860.6980147859046046No Hit
TCGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1840.6905092505722971No Hit
AACCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1700.637970503246144No Hit
GACCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1640.6154538972492213No Hit
GTGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1570.5891845235861448No Hit
CACCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1560.585431755919991No Hit
ATGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1440.5403985439261455No Hit
TGGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1440.5403985439261455No Hit
CGCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1430.5366457762599917No Hit
GGGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1380.5178819379292228No Hit
ACCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1370.514129170263069No Hit
CGGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1330.4991180995984538No Hit
AGGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1320.4953653319323001No Hit
GCGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1300.4878597965999925No Hit
CGGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1260.47284872593537736No Hit
TAGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1240.4653431906030698No Hit
CCCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1210.4540848876046084No Hit
TAGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1180.44282658460614704No Hit
GTGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1160.4353210492738395No Hit
CCGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1160.4353210492738395No Hit
AAGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1150.4315682816076857No Hit
GTGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1140.42781551394153194No Hit
CGGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1130.4240627462753781No Hit
ATGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1110.4165572109430705No Hit
GCGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1090.40905167561076294No Hit
CTGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1040.390287837279994No Hit
GTGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1040.390287837279994No Hit
TTGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1010.37902953428153263No Hit
TCGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1010.37902953428153263No Hit
CAGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG990.37152399894922505No Hit
TAGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA980.36777123128307126No Hit
ATGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG930.3490073929523023No Hit
TTGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA910.34150185761999474No Hit
CCGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG900.33774908995384095No Hit
ACGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG890.33399632228768716No Hit
GGGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA880.3302435546215334No Hit
TAGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG860.3227380192892258No Hit
TGGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG850.318985251623072No Hit
CTGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG840.3152324839569182No Hit
CAGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG810.30397418095845685No Hit
TCGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA800.30022141329230306No Hit
AAGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG760.2852103426276879No Hit
ATGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA740.27770480729538033No Hit
AAGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA680.2551882012984576No Hit
AAGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG650.24392989829999626No Hit
GAGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA610.2289188276353811No Hit
ACGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA540.20264945397230455No Hit
CTGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA520.195143918639997No Hit
GCGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA520.195143918639997No Hit
CAGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA490.18388561564153563No Hit
GAGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG490.18388561564153563No Hit
ATGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA490.18388561564153563No Hit
GAGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG480.18013284797538184No Hit
CAGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA460.17262731264307427No Hit
GCGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG410.15386347431230532No Hit
CAGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG400.15011070664615153No Hit
CCGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA400.15011070664615153No Hit
TTGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA390.14635793897999774No Hit
TAGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA380.14260517131384395No Hit
GAGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA370.13885240364769016No Hit
CCGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA370.13885240364769016No Hit
GTGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA360.13509963598153638No Hit
GAGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG350.1313468683153826No Hit
ATCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA340.1275941006492288No Hit
GGGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG320.12008856531692123No Hit
GGCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA310.11633579765076744No Hit
TGCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA310.11633579765076744No Hit
GCCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA310.11633579765076744No Hit
ACGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA300.11258302998461366No Hit
CGCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA300.11258302998461366No Hit
GAGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA300.11258302998461366No Hit
GTCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA290.10883026231845988No Hit
AGCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA290.10883026231845988No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)