FastQCFastQC Report
Fri 2 Sep 2022
EGAF00005002499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265768
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6860.2581198639414828No Hit
GCGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA4980.18738147557267995No Hit
CCGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA4770.17947984708467535No Hit
GCGTATCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4720.17759850696848378No Hit
CCGTATCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4520.17007314650371752No Hit
CGGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACCCAAGTTGGATGAG4500.1693206104572409No Hit
GTGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA4370.16442912615514282No Hit
GCGTATCGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG4290.16141898196923632No Hit
TCGTATCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4290.16141898196923632No Hit
CGGTATCGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG4260.16029017789952138No Hit
CAGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA4120.155022425574185No Hit
GGGTATCGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG4050.1523885494115168No Hit
CTGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA4010.15088347731856355No Hit
ACGTATCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4000.15050720929532524No Hit
GAGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA3990.15013094127208693No Hit
TGGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA3920.14749706510941873No Hit
CGGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT3880.14599199301646548No Hit
CGGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC3720.13997170464465247No Hit
GGGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA3680.13846663255169922No Hit
CCGTATCGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3650.13733782848198428No Hit
AGGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA3650.13733782848198428No Hit
GAGTATCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3610.13583275638903103No Hit
CAGTATCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3560.13395141627283946No Hit
TCGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA3560.13395141627283946No Hit
GCGTATCGAAGGAGCCAGGGTTTTCCCAGTCACGACCTCTGAGCTGAATG3510.1320700761566479No Hit
CTGTATCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3500.13169380813340958No Hit
CGGTATCGACGAGGCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC3490.13131754011017127No Hit
GCGTATCGACGAGGCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC3440.1294361999939797No Hit
TGGTATCGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3440.1294361999939797No Hit
CGGTATCGACTCAGCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT3430.1290599319707414No Hit
GAGTATCGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3420.12868366394750308No Hit
GCGTATCGACTCAGCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT3390.12755485987778814No Hit
TCGTATCGAAGGAGCCAGGGTTTTCCCAGTCACGACCTCTGAGCTGAATG3350.1260497877848349No Hit
GTGTATCGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3310.12454471569188165No Hit
GGGTATCGACGAGGCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC3300.12416844766864332No Hit
GCGTATCGATGTTGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3270.12303964359892838No Hit
AGGTATCGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3260.12266337557569008No Hit
CCGTATCGAAGGAGCCAGGGTTTTCCCAGTCACGACCTCTGAGCTGAATG3230.12153457150597513No Hit
GCGTATCGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG3200.12040576743626019No Hit
GGGTATCGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG3180.11965323138978357No Hit
GCGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACCCAAGTTGGATGAG3170.11927696336654525No Hit
GGGTATCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3170.11927696336654525No Hit
CCGTATCGACTCAGCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT3160.11890069534330695No Hit
TGGTATCGACTCAGCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT3120.11739562325035369No Hit
GTGTATCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3100.11664308720387706No Hit
TTGTATCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3100.11664308720387706No Hit
GCGTATCGACTCAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3050.1147617470876855No Hit
GCGTATCGATGTTGCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC3030.11400921104120887No Hit
CGGTATCGACTCAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT2970.11175160290177899No Hit
CGGTATCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT2950.11099906685530236No Hit
AAGTATCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT2890.1087414587158725No Hit
TCGTATCGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG2890.1087414587158725No Hit
CCGTATCGACGAGGCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC2890.1087414587158725No Hit
CGGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACCTCAGTTTGGTATA2880.10836519069263417No Hit
GTGTATCGATGCACCCAGGGTTTTCCCAGTCACGACCTCAGTTTGGTATA2880.10836519069263417No Hit
ACGTATCGAAGGAGCCAGGGTTTTCCCAGTCACGACCTCTGAGCTGAATG2850.10723638662291923No Hit
ATGTATCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT2840.10686011859968093No Hit
CGGTATCGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG2830.1064838505764426No Hit
GGGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACCCAAGTTGGATGAG2810.105731314529966No Hit
CCGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT2800.10535504650672767No Hit
GAGTATCGACGAGGCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC2800.10535504650672767No Hit
TAGTATCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT2790.10497877848348937No Hit
GTGTATCGACGAGGCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC2790.10497877848348937No Hit
GTGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC2780.10460251046025104No Hit
GCGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC2750.1034737063905361No Hit
GTGTATCGAGGAATCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG2740.1030974383672978No Hit
TGGTATCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT2720.10234490232082116No Hit
GTGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACCCAAGTTGGATGAG2720.10234490232082116No Hit
GCGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT2700.10159236627434454No Hit
CCGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC2700.10159236627434454No Hit
GCGTATCGACTCAGCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG2700.10159236627434454No Hit
CCGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACCCAAGTTGGATGAG2700.10159236627434454No Hit
TAGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA2680.10083983022786791No Hit
ACGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA2670.1004635622046296No Hit
GAGTATCGATAAGCCCAGGGTTTTCCCAGTCACGACCCAAGTTGGATGAG2660.1000872941813913No Hit
ACGTATCGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG2660.1000872941813913No Hit
TCGTATCGACTCAGCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT2660.1000872941813913No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)