FastQCFastQC Report
Sat 3 Sep 2022
EGAF00005002505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381836
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGCTCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT11030.2888674718989304No Hit
TGAGCTCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT9750.25534522674656135No Hit
AGAGCTCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT8660.22679893985899707No Hit
GGAGCTCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT8550.22391812191621535No Hit
CCAGCTCGATGCACCCAGGGTTTTCCCAGTCACGACTCCAGAAGGCAAGA7590.19877643805193854No Hit
CCAGCTCGATGCACCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC7130.18672938120030588No Hit
GGCCTGCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT7090.18568181103929435No Hit
TTAGCTCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT6900.18070585277448958No Hit
TTAGCTCGACTCAGCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC6810.17834881991221363No Hit
ACCCTGCGACAACTCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA6580.1723252914863973No Hit
TCAGCTCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT6570.17206339894614442No Hit
TGAGCTCGACGAGGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA6510.17049204370462712No Hit
CAAGCTCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT6460.1691825810033627No Hit
CCAGCTCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT6440.16865879592285693No Hit
GTAGCTCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT6360.16656365560083386No Hit
CGAGCTCGACGAGGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA6320.16551608543982232No Hit
CAAGCTCGATGCACCCAGGGTTTTCCCAGTCACGACTCCAGAAGGCAAGA6300.16499230035931656No Hit
CTAGCTCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT6280.1644685152788108No Hit
ATAGCTCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT6050.15844498685299446No Hit
CAAGCTCGATGCACCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC5980.1566117390712243No Hit
GGAGCTCGATGTTGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5910.15477849128945412No Hit
ACAGCTCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT5820.15242145842717816No Hit
TAAGCTCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT5810.15215956588692528No Hit
CCAGCTCGATGCACCCAGGGTTTTCCCAGTCACGACCCAAGTTGGATGAG5790.1516357808064195No Hit
TTAGCTCGACTCAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT5740.1503263181051551No Hit
AAAGCTCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT5680.1487549628636378No Hit
AGAGCTCGACGAGGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5590.14639793000136184No Hit
GGAGCTCGACGAGGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5560.14561225238060319No Hit
TGAGCTCGACTCAGCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC5550.1453503598403503No Hit
GCAGCTCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT5540.14508846730009745No Hit
GGAGCTCGACTCAGCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC5530.14482657475984453No Hit
GACCTGCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT5440.14246954189756858No Hit
CTAGCTCGACTCAGCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC5430.14220764935731572No Hit
GCAGCTCGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG5320.139326831414534No Hit
TGAGCTCGAGGAATCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC5290.13854115379377535No Hit
TCAGCTCGACTCAGCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC5250.13749358363276382No Hit
TGCCTGCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT5200.1361841209314994No Hit
CGAGCTCGACTCAGCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC5190.1359222283912465No Hit
CTAGCTCGATGCACCCAGGGTTTTCCCAGTCACGACTCCAGAAGGCAAGA5060.132517625367959No Hit
TAAGCTCGAGGAATCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC5060.132517625367959No Hit
AACCTGCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT5040.13199384028745326No Hit
CTAGCTCGATGCACCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC4990.13068437758618884No Hit
GGCCTGCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG4980.13042248504593595No Hit
TGCCTGCGACAACTCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4970.13016059250568307No Hit
GAAGCTCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4950.1296368074251773No Hit
TCCCTGCGACAACTCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4850.12701788202264847No Hit
GTAGCTCGACTCAGCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC4850.12701788202264847No Hit
AAAGCTCGAGGAATCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC4740.12413706407986674No Hit
CTAGCTCGACGAGGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4720.12361327899936099No Hit
AGAGCTCGACTCAGCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC4700.12308949391885522No Hit
ATCCTGCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4690.12282760137860232No Hit
CGAGCTCGATGCACCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC4620.12099435359683214No Hit
TTAGCTCGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG4620.12099435359683214No Hit
GCAGCTCGACTCAGCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC4620.12099435359683214No Hit
AACCTGCGACAACTCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4600.12047056851632637No Hit
GGAGCTCGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG4580.1199467834358206No Hit
CGAGCTCGATGCACCCAGGGTTTTCCCAGTCACGACTCCAGAAGGCAAGA4570.11968489089556773No Hit
TTAGCTCGACGAGGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4560.11942299835531484No Hit
GCCCTGCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4550.11916110581506197No Hit
CAAGCTCGATGCACCCAGGGTTTTCCCAGTCACGACCCAAGTTGGATGAG4530.11863732073455621No Hit
TTCCTGCGATGCACCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4510.11811353565405042No Hit
AGCCTGCGATGTTGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4500.11785164311379755No Hit
GTCCTGCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4480.11732785803329178No Hit
TCAGCTCGACGAGGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4430.11601839533202736No Hit
GGAGCTCGACTCAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4430.11601839533202736No Hit
CCAGCTCGATGCACCCAGGGTTTTCCCAGTCACGACCTCCGCACAACAGT4410.11549461025152159No Hit
AGAGCTCGAGGAATCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC4400.11523271771126872No Hit
TTCCTGCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4400.11523271771126872No Hit
TAAGCTCGACTCAGCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC4390.11497082517101583No Hit
GAAGCTCGACTCAGCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC4380.11470893263076294No Hit
TACCTGCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4370.11444704009051007No Hit
GACCTGCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG4370.11444704009051007No Hit
TCAGCTCGACTCAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4370.11444704009051007No Hit
CCAGCTCGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG4350.1139232550100043No Hit
GCAGCTCGATAAGCCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC4350.1139232550100043No Hit
GAAGCTCGATGTTGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4350.1139232550100043No Hit
TGCCTGCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4350.1139232550100043No Hit
CAAGCTCGACTCAGCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC4330.11339946992949854No Hit
AGCCTGCGACAACTCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4310.11287568484899277No Hit
CCAGCTCGACTCAGCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC4310.11287568484899277No Hit
GGCCTGCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG4270.11182811468798122No Hit
GTCCTGCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4250.11130432960747545No Hit
TGCCTGCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG4250.11130432960747545No Hit
ATCCTGCGACAACTCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4240.11104243706722258No Hit
ATAGCTCGACTCAGCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC4220.11051865198671681No Hit
GCAGCTCGATGTTGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4190.10973297436595816No Hit
GACCTGCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4190.10973297436595816No Hit
TCAGCTCGAGGAATCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC4170.1092091892854524No Hit
CGAGCTCGACTCAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4160.1089472967451995No Hit
AGCCTGCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4110.1076378340439351No Hit
CACCTGCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4090.10711404896342934No Hit
AGCCTGCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4070.10659026388292357No Hit
TGAGCTCGACTCAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4070.10659026388292357No Hit
CTAGCTCGATGCACCCAGGGTTTTCCCAGTCACGACCCAAGTTGGATGAG4050.10606647880241778No Hit
GGCCTGCGATGTTGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4020.10528080118165914No Hit
TTCCTGCGACAACTCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4000.10475701610115339No Hit
GTAGCTCGATGTTGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3980.10423323102064762No Hit
GACCTGCGACAACTCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3980.10423323102064762No Hit
GAAGCTCGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG3940.10318566085963607No Hit
GCAGCTCGATAAGCCCAGGGTTTTCCCAGTCACGACCTCCGCACAACAGT3920.1026618757791303No Hit
CGAGCTCGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3920.1026618757791303No Hit
CTAGCTCGACTCAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3920.1026618757791303No Hit
TAAGCTCGAGGAATCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3910.10239998323887743No Hit
TTAGCTCGAGGAATCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC3900.10213809069862453No Hit
TAAGCTCGACGAGGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3880.10161430561811877No Hit
ACCCTGCGATGCACCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3870.1013524130778659No Hit
CCAGCTCGATGCACCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG3870.1013524130778659No Hit
GGAGCTCGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3850.10082862799736013No Hit
TCCCTGCGATGCACCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3840.10056673545710725No Hit
ATAGCTCGACGAGGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3830.10030484291685435No Hit
TCCCTGCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3820.10004295037660148No Hit
GAAGCTCGACTCAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3820.10004295037660148No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)