FastQCFastQC Report
Fri 2 Sep 2022
EGAF00005002509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319454
Sequences flagged as poor quality0
Sequence length251
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATATGATGCACCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA8320.2604443832288843No Hit
GGTATATGATGCACCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA7860.24604481396382577No Hit
TGTATATGATGCACCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA7760.24291447281924783No Hit
GCTATATGACGAGGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA7540.2360277223011764No Hit
TCTATATGACGAGGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA7400.23164524469876727No Hit
GTTATATGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6920.21661960720479317No Hit
AGTATATGATGCACCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA6890.21568050486141982No Hit
GGTATATGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6110.1912638439337119No Hit
TCTATATGATGCACCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5960.186568332216845No Hit
GCTATATGATGCACCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5950.1862552981023872No Hit
GTTATATGACGAGGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5940.1859422639879294No Hit
TTTATATGATGCACCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5790.1812467522710625No Hit
TTTATATGACGAGGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5760.1803076499276891No Hit
TGTATATGACGAGGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5670.177490342897569No Hit
CATATATGATGCACCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5570.17436000175299105No Hit
GTTATATGATGCACCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5450.17060359237949752No Hit
ATTATATGATGCACCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5430.16997752415058193No Hit
CTTATATGATGCACCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5400.16903842180720854No Hit
AATATATGATGCACCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5370.16809931946383516No Hit
GGTATATGACGAGGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5280.16528201243371504No Hit
ACTATATGATGCACCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5190.16246470540359487No Hit
TGTATATGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT5110.15996043248793254No Hit
GGTATATGATGTTGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA5110.15996043248793254No Hit
TATATATGATGCACCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5100.15964739837347475No Hit
CCTATATGACGAGGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5070.15870829603010136No Hit
GGTATATGACTCAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4980.15589098899998122No Hit
GATATATGATGCACCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4890.1530736819698611No Hit
GGTATATGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4800.15025637493974092No Hit
TCTATATGACGAGGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA4800.15025637493974092No Hit
CGTATATGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4790.14994334082528313No Hit
GCTATATGACGAGGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA4780.14963030671082533No Hit
CTTATATGACAACTCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT4780.14963030671082533No Hit
GTTATATGATGTTGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA4780.14963030671082533No Hit
TTTATATGAAGGAGCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT4720.1477521020240786No Hit
CATATATGAAGGAGCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT4700.147126033795163No Hit
CCTATATGATGCACCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4620.14462176087950065No Hit
GTTATATGACTCAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4610.14430872676504286No Hit
CTTATATGAAGGAGCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT4570.14305659030721168No Hit
TGTATATGATAAGCCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA4570.14305659030721168No Hit
TTTATATGACAACTCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT4560.1427435561927539No Hit
AGTATATGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4550.1424305220782961No Hit
TGTATATGACTCAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4520.1414914197349227No Hit
GATATATGACGAGGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4500.14086535150600712No Hit
CGTATATGACAACTCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT4470.13992624916263374No Hit
TGTATATGACTCAGCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC4440.13898714681926036No Hit
GGTATATGACTCAGCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC4370.1367959080180558No Hit
ATTATATGACAACTCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT4350.13616983978914024No Hit
TGTATATGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4340.13585680567468245No Hit
TTTATATGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA4340.13585680567468245No Hit
TTTATATGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT4280.13397860098793565No Hit
TATATATGAAGGAGCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT4270.13366556687347786No Hit
TTTATATGACTCAGCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC4260.13335253275902006No Hit
CATATATGAGGAATCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT4180.13084825984335774No Hit
GTTATATGACTCAGCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC4150.12990915749998436No Hit
GTTATATGATGTTGCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT4150.12990915749998436No Hit
GATATATGACTCAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4110.12865702104215315No Hit
GATATATGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA4100.12834398692769539No Hit
ATTATATGAAGGAGCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT4100.12834398692769539No Hit
CATATATGACAACTCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT4100.12834398692769539No Hit
TTTATATGACTCAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4100.12834398692769539No Hit
AATATATGAAGGAGCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT4070.127404884584322No Hit
GTTATATGACGAGGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA4070.127404884584322No Hit
CTTATATGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT4050.12677881635540642No Hit
TGTATATGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA4050.12677881635540642No Hit
TATATATGACTCAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4040.12646578224094862No Hit
CATATATGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT4030.12615274812649083No Hit
GCTATATGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA4010.12552667989757524No Hit
GGTATATGACGAGGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA3990.12490061166865965No Hit
GTTATATGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3970.12427454343974408No Hit
ATTATATGACTCAGCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC3960.12396150932528628No Hit
GTTATATGACAACTCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT3960.12396150932528628No Hit
TATATATGACTCAGCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC3960.12396150932528628No Hit
CTTATATGACGAGGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3940.12333544109637068No Hit
TATATATGACGAGGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3930.12302240698191289No Hit
GGTATATGATGTTGCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT3930.12302240698191289No Hit
CATATATGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT3930.12302240698191289No Hit
TTTATATGACGAGGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA3920.12270937286745509No Hit
TATATATGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT3900.1220833046385395No Hit
TGTATATGACAACTCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT3890.12177027052408171No Hit
ACTATATGACGAGGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3880.12145723640962391No Hit
CGTATATGAAGGAGCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT3860.12083116818070834No Hit
AGTATATGACTCAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3840.12020509995179275No Hit
CCTATATGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT3820.11957903172287716No Hit
TGTATATGAAGGAGCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT3820.11957903172287716No Hit
CGTATATGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA3820.11957903172287716No Hit
TGTATATGACGAGGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA3810.11926599760841937No Hit
TCTATATGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3800.11895296349396157No Hit
AATATATGACAACTCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT3790.11863992937950378No Hit
ATTATATGACTCAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3770.1180138611505882No Hit
GCTATATGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3760.11770082703613038No Hit
GTTATATGATGTTGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3730.11676172469275702No Hit
CTTATATGAGGAATCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT3720.11644869057829922No Hit
CGTATATGACGAGGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3710.11613565646384143No Hit
CTTATATGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT3710.11613565646384143No Hit
AGTATATGACTCAGCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC3700.11582262234938363No Hit
TGTATATGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT3640.11394441766263688No Hit
CATATATGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3630.11363138354817909No Hit
TTTATATGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3620.1133183494337213No Hit
GGTATATGATAAGCCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA3620.1133183494337213No Hit
TCTATATGACAACTCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT3570.11175317886143231No Hit
ATTATATGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA3560.11144014474697451No Hit
TGTATATGATAAGCCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA3530.11050104240360115No Hit
CGTATATGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT3530.11050104240360115No Hit
ATTATATGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT3510.10987497417468556No Hit
AATATATGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT3500.10956194006022776No Hit
TTTATATGATAAGCCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA3480.10893587183131218No Hit
TATATATGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3470.10862283771685438No Hit
TATATATGACAACTCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT3470.10862283771685438No Hit
AGTATATGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA3460.10830980360239659No Hit
CCTATATGACGAGGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA3440.10768373537348101No Hit
TGTATATGATAAGCCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC3420.10705766714456542No Hit
GGTATATGATGTTGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3420.10705766714456542No Hit
CTTATATGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA3390.10611856480119203No Hit
CTTATATGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3350.10486642834336085No Hit
AGTATATGAAGGAGCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT3340.10455339422890306No Hit
CCTATATGAAGGAGCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT3340.10455339422890306No Hit
ATTATATGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3320.10392732599998748No Hit
CCTATATGAGGAATCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT3320.10392732599998748No Hit
TATATATGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3320.10392732599998748No Hit
AGTATATGATAAGCCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA3320.10392732599998748No Hit
CCTATATGACAACTCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT3310.10361429188552969No Hit
TTTATATGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT3310.10361429188552969No Hit
ACTATATGACAACTCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT3300.1033012577710719No Hit
AGTATATGACGAGGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3300.1033012577710719No Hit
TTTATATGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3290.1029882236566141No Hit
CATATATGACGAGGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3270.10236215542769851No Hit
AGTATATGACAACTCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT3270.10236215542769851No Hit
GATATATGACTCAGCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC3270.10236215542769851No Hit
TTTATATGATAAGCCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA3260.10204912131324072No Hit
TATATATGATAAGCCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA3240.10142305308432514No Hit
AATATATGACTCAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3240.10142305308432514No Hit
ACTATATGAAGGAGCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT3210.10048395074095173No Hit
GGTATATGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3200.10017091662649395No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)