FastQCFastQC Report
Sun 4 Sep 2022
EGAF00005002515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894150
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACAAGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG27370.3061007660907007No Hit
TTAACAAGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG20330.22736677291282223No Hit
GCTATATGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG19150.2141698820108483No Hit
CATATATGAGGAATCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG18920.21159760666554828No Hit
CTTATATGAGGAATCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG18710.20924900743723088No Hit
CCTATATGAGGAATCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG18580.20779511267684392No Hit
TCTATATGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG18430.20611754179947436No Hit
TTAACAAGAGGAATCCAGGGTTTTCCCAGTCACGACCACAATCTCCTTCA16180.1809539786389308No Hit
CGTATATGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG15960.17849354135212211No Hit
GGTATATGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG15890.177710674942683No Hit
TGTATATGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG15570.17413185707096127No Hit
TTAACAAGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG15470.1730134764860482No Hit
TGTATATGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG14940.16708605938600907No Hit
TCTATATGAGGAATCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG14890.16652686909355252No Hit
GTTATATGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG14400.1610468042274786No Hit
TTTATATGAGGAATCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG14270.15959290946709165No Hit
TGTATATGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG14200.15881004305765253No Hit
AGTATATGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG14190.1586982049991612No Hit
ATAACAAGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG13720.1534418162500699No Hit
GGTATATGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG13440.15031035061231338No Hit
TATATATGAGGAATCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG13390.14975116031985686No Hit
CGTATATGAGGAATCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG13320.14896829391041772No Hit
TTTATATGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG13040.1458368282726612No Hit
GCTATATGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG12110.13543588883296986No Hit
GCTATATGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG11920.13331096572163506No Hit
TTAACAAGATGTTGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG11830.13230442319521332No Hit
TCTATATGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG11790.13185707096124813No Hit
TTAACAAGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG11790.13185707096124813No Hit
GCTATATGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG11770.1316333948442655No Hit
TTTATATGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG11760.1315215567857742No Hit
TGTATATGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA11750.13140971872728288No Hit
CATATATGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG11720.131074204551809No Hit
GTAACAAGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG11710.13096236649331766No Hit
TTAACAAGAGGAATCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG11710.13096236649331766No Hit
TCTATATGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG11670.13051501425935247No Hit
CCTATATGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG11640.13017950008387855No Hit
CTAACAAGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG11540.12906111949896548No Hit
TTTATATGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG11500.1286137672650003No Hit
TCAACAAGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG11470.12827825308952637No Hit
CATATATGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG11450.12805457697254377No Hit
CTTATATGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG11410.12760722473857852No Hit
TTAACAAGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG11360.12704803444612203No Hit
TCTATATGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG11320.12660068221215678No Hit
TAAACAAGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG11260.12592965386120897No Hit
ATAACAAGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG11160.12481127327629593No Hit
CTAACAAGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG11000.12302186434043505No Hit
TGTATATGACTCAGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG10980.12279818822345243No Hit
CCTATATGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG10950.12246267404797853No Hit
CCTATATGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG10870.12156796958004809No Hit
GGTATATGACTCAGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG10860.12145613152155679No Hit
AATATATGAGGAATCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG10790.12067326511211766No Hit
ATTATATGAGGAATCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG10780.12056142705362635No Hit
ACTATATGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG10770.12044958899513505No Hit
ACTATATGAGGAATCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG10750.12022591287815243No Hit
CTTATATGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG10710.11977856064418722No Hit
GTAACAAGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG10710.11977856064418722No Hit
TGAACAAGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG10700.11966672258569593No Hit
CTTATATGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG10690.1195548845272046No Hit
GATATATGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG10690.1195548845272046No Hit
TATATATGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG10650.1191075322932394No Hit
TATATATGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG10620.11877201811776547No Hit
TGAACAAGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG10550.11798915170832633No Hit
GGAACAAGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG10540.11787731364983503No Hit
CATATATGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG10460.11698260918190459No Hit
GCTATATGAGGAATCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG10400.11631158083095676No Hit
GTTATATGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG10390.11619974277246546No Hit
TGTATATGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG10320.11541687636302633No Hit
AATATATGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG10300.11519320024604372No Hit
TTTATATGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA10280.11496952412906113No Hit
ACTATATGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG10250.1146340099535872No Hit
GTTATATGAGGAATCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG10100.11295643907621764No Hit
ATTATATGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG10090.11284460101772632No Hit
GATATATGAGGAATCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG10070.11262092490074373No Hit
GTTATATGACTCAGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG10020.1120617346082872No Hit
AGTATATGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG9990.11172622043281329No Hit
GATATATGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG9980.11161438237432199No Hit
GTTATATGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG9890.11060783984790024No Hit
GGTATATGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG9870.11038416373091763No Hit
CTTATATGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG9840.11004864955544372No Hit
TTTATATGACTCAGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG9830.10993681149695242No Hit
ATTATATGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG9770.10926578314600459No Hit
TATATATGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG9760.10915394508751329No Hit
CTAACAAGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG9750.10904210702902196No Hit
TCAACAAGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG9700.10848291673656546No Hit
GTTATATGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG9700.10848291673656546No Hit
GGTATATGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG9700.10848291673656546No Hit
CGAACAAGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG9630.10770005032712633No Hit
TGTATATGAGGAATCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG9510.10635799362523066No Hit
GTAACAAGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG9480.10602247944975676No Hit
TATATATGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA9450.10568696527428283No Hit
AGTATATGACTCAGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG9390.105015936923335No Hit
GGAACAAGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG9350.10456858468936979No Hit
GTAACAAGATGTTGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG9340.10445674663087849No Hit
CCTATATGATGCACCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG9290.10389755633842196No Hit
CTAACAAGATGTTGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG9280.10378571827993066No Hit
GGTATATGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG9250.10345020410445675No Hit
ATAACAAGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG9230.10322652798747413No Hit
TATATATGACTCAGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG9200.10289101381200022No Hit
TGAACAAGATGTTGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG9200.10289101381200022No Hit
CTAACAAGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG9180.10266733769501762No Hit
CGTATATGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG9180.10266733769501762No Hit
GGTATATGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA9150.1023318235195437No Hit
TTAACAAGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG9130.10210814740256109No Hit
GGTATATGACGAGGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG9130.10210814740256109No Hit
AATATATGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG9120.1019963093440698No Hit
TGAACAAGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG9090.10166079516859587No Hit
ATAACAAGAGGAATCCAGGGTTTTCCCAGTCACGACCACAATCTCCTTCA9090.10166079516859587No Hit
AGTATATGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA9040.10110160487613935No Hit
TCAACAAGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG8990.10054241458368282No Hit
GTTATATGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG8980.10043057652519152No Hit
TAAACAAGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG8980.10043057652519152No Hit
CTAACAAGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG8960.10020690040820893No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)