FastQCFastQC Report
Sun 4 Sep 2022
EGAF00005002525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705480
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT20300.28774734932244717No Hit
TTATGTCGACTCAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT19350.2742813403640075No Hit
TTATGTCGATGTTGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT18620.26393377558541703No Hit
TTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT17860.2531609684186653No Hit
TTATGTCGATGTTGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG17790.2521687361796224No Hit
TTATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG16120.22849690990531268No Hit
GTATGTCGACTCAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT15920.22566196065090435No Hit
TTATCCTGACGAGGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG14700.20836877019901343No Hit
TCATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG14510.2056755684073255No Hit
TTATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG14490.20539207348188468No Hit
TTATGTCGATGTTGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG14330.203124114078358No Hit
TTATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT14250.20199013437659463No Hit
TCATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT14230.2017066394511538No Hit
TTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG13900.19702897318138007No Hit
TTATGTCGACAACTCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG13840.19617848840505756No Hit
TTATCCTGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT13800.1956114985541759No Hit
ATATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT13610.19291829676248795No Hit
ATATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT13200.18710665079095085No Hit
GTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT12700.18001927765492998No Hit
TGATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT12560.17803481317684414No Hit
ATATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG12540.1777513182514033No Hit
ACATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG12310.1744911266088337No Hit
ACATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT12280.17406588422067246No Hit
TAATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT12260.17378238929523163No Hit
TCATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT12160.17236491466802742No Hit
GTATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT12060.17094744004082327No Hit
TAATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG12030.17052219765266202No Hit
CTATGTCGACAACTCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG11960.1695299654136191No Hit
TAATGTCGATGTTGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT11860.16811249078641494No Hit
TTATGTCGACTCAGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG11850.16797074332369452No Hit
CTATGTCGACTCAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT11820.16754550093553325No Hit
TTATGTCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT11730.1662697737710495No Hit
CTATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT11640.16499404660656575No Hit
TAATGTCGACTCAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT11310.16031638033679196No Hit
ATATGTCGACAACTCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG11270.1597493904859103No Hit
ATATGTCGATGTTGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG11230.15918240063502864No Hit
CTATGTCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT11190.15861541078414698No Hit
GTATGTCGATGTTGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT11150.1580484209332653No Hit
ATATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT11050.15663094630606111No Hit
TAATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT10980.1556387140670182No Hit
ATATGTCGATGTTGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT10890.15436298690253444No Hit
TAATGTCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT10880.15422123943981403No Hit
TGATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG10810.15322900720077112No Hit
TAATGTCGATGTTGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG10760.15252026988716902No Hit
TAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT10530.14926007824459941No Hit
CAATGTCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT10530.14926007824459941No Hit
TCATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG10520.149118330781879No Hit
TCATGTCGACAACTCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG10360.14685037137835233No Hit
GTATGTCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT10340.1465668764529115No Hit
TCATGTCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT10310.14614163406475025No Hit
ATATGTCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT10290.1458581391393094No Hit
TCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT10280.14571639167658898No Hit
TTATCCTGACTCAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT10220.1448659069002665No Hit
TCATGTCGACTCAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT10110.1433066848103419No Hit
AAATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG10100.14316493734762148No Hit
AAATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT10090.14302318988490106No Hit
ATATGTCGACTCAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT9970.14132222033225605No Hit
TGATGTCGACTCAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT9970.14132222033225605No Hit
GTATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG9970.14132222033225605No Hit
GTATGTCGATGTTGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG9860.13976299824233146No Hit
AAATGTCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT9800.13891251346600897No Hit
CTATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG9760.13834552361512728No Hit
GTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG9760.13834552361512728No Hit
TAATGTCGACAACTCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG9730.13792028122696603No Hit
ATATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG9710.1376367863015252No Hit
TTATCCTGATAAGCCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT9700.1374950388388048No Hit
TGATGTCGATGTTGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT9660.13692804898792313No Hit
TTATCCTGAAGGAGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG9640.1366445540624823No Hit
TTATGTCGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG9590.1359358167488802No Hit
TTATGTCGACGAGGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG9580.13579406928615978No Hit
GTATGTCGATGTTGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG9480.1343765946589556No Hit
GTATCCTGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT9410.13338436241991267No Hit
GTATGTCGACTCAGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG9410.13338436241991267No Hit
GTATGTCGACAACTCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG9360.1326756251063106No Hit
CCATGTCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT9180.1301241707773431No Hit
TGATGTCGATGTTGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG9140.12955718092646143No Hit
ATATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG9100.12899019107557974No Hit
CTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT9070.1285649486874185No Hit
TAATCCTGACGAGGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG9060.12842320122469808No Hit
GTATCCTGACGAGGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG9030.12799795883653683No Hit
CAATGTCGACAACTCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG9010.127714463911096No Hit
TAATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG9010.127714463911096No Hit
TGATCCTGACGAGGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG8870.12572999943301014No Hit
TGATCCTGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8840.1253047570448489No Hit
TTATGTCGACAACTCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8750.12402902988036513No Hit
TCATGTCGATGTTGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8720.12360378749220388No Hit
TGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8720.12360378749220388No Hit
GAATGTCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8710.12346204002948347No Hit
CCATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8660.12275330271588139No Hit
ACATGTCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8650.12261155525316098No Hit
TTATGTCGATGCACCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8570.12147757555139763No Hit
ACATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8550.1211940806259568No Hit
TAATGTCGATGTTGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG8500.1204853433123547No Hit
TCATGTCGATGTTGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG8490.12034359584963429No Hit
TGATGTCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8460.11991835346147305No Hit
TAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG8350.11835913137154845No Hit
CCATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG8330.11807563644610762No Hit
TAATCCTGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8330.11807563644610762No Hit
TTATGTCGATGCACCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG8220.11651641435618301No Hit
TCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG8200.11623291943074218No Hit
AAATGTCGACAACTCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG8190.11609117196802177No Hit
ATATCCTGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8130.11524068719169928No Hit
CTATGTCGATGTTGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG8130.11524068719169928No Hit
ATATCCTGACGAGGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG8130.11524068719169928No Hit
CTATGTCGATGTTGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8080.11453194987809717No Hit
GCATGTCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8080.11453194987809717No Hit
CTATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8060.11424845495265634No Hit
CTATGTCGATGCACCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8050.11410670748993593No Hit
CAATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG8030.1138232125644951No Hit
CGATGTCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8020.11368146510177468No Hit
CCATGTCGACAACTCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG8000.11339797017633384No Hit
ATATGTCGATGTTGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG7980.113114475250893No Hit
GGATGTCGACTCAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7950.11268923286273176No Hit
TGATCCTGAAGGAGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG7950.11268923286273176No Hit
GAATGTCGACTCAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7940.11254748540001135No Hit
ATATGTCGACAACTCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7890.11183874808640924No Hit
TTATGTCGAAGGAGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG7810.11070476838464592No Hit
CTATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG7770.11013777853376425No Hit
CAATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7700.10914554629472131No Hit
TTATCCTGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7680.10886205136928048No Hit
ACATGTCGACAACTCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG7650.10843680898111924No Hit
GCATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7650.10843680898111924No Hit
CAATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7630.1081533140556784No Hit
GTATCCTGATGCACCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG7600.10772807166751716No Hit
CTATGTCGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG7590.10758632420479675No Hit
CTATCCTGAAGGAGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG7580.10744457674207633No Hit
GTATCCTGACTCAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7560.10716108181663547No Hit
TTATGTCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7520.10659409196575381No Hit
AGATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7470.10588535465215172No Hit
GCATCCTGATGCACCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG7450.10560185972671089No Hit
TGATCCTGATAAGCCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7450.10560185972671089No Hit
TTATCCTGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA7450.10560185972671089No Hit
AGATGTCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7440.10546011226399048No Hit
GTATGTCGATGCACCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7430.10531836480127006No Hit
TTATCCTGATGCACCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG7410.10503486987582923No Hit
GGATGTCGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7400.10489312241310879No Hit
ACATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG7400.10489312241310879No Hit
TGATGTCGATGTTGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG7400.10489312241310879No Hit
CTATGTCGACAACTCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7380.10460962748766796No Hit
GTATGTCGATGCACCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG7380.10460962748766796No Hit
TTATGTCGACTCAGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG7360.10432613256222713No Hit
TGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG7340.1040426376367863No Hit
CTATGTCGATGCACCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG7330.10390089017406588No Hit
TTATGTCGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG7320.10375914271134547No Hit
GTATCCTGAAGGAGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG7310.10361739524862505No Hit
CGATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7280.1031921528604638No Hit
CTATGTCGACTCAGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG7220.10234166808414129No Hit
CTATGTCGAAGGAGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG7210.10219992062142087No Hit
GTATCCTGATGCACCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7190.10191642569598004No Hit
CTATCCTGATGCACCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG7190.10191642569598004No Hit
CTATGTCGAGGAATCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG7190.10191642569598004No Hit
TCATGTCGATGTTGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG7180.10177467823325961No Hit
CCATGTCGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG7180.10177467823325961No Hit
TCATCCTGACGAGGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG7180.10177467823325961No Hit
TGATCCTGACTCAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7170.1016329307705392No Hit
CAATGTCGAAGGAGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG7160.10149118330781878No Hit
ATATGTCGAAGGAGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG7150.10134943584509837No Hit
CAATGTCGACTCAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7150.10134943584509837No Hit
TCATCCTGAAGGAGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG7110.10078244599421671No Hit
GGATCCTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7110.10078244599421671No Hit
GCATGTCGACTCAGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7100.10064069853149629No Hit
CTATCCTGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7100.10064069853149629No Hit
CTATCCTGACGAGGCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG7090.10049895106877588No Hit
AAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7090.10049895106877588No Hit
TCATGTCGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG7090.10049895106877588No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)