FastQCFastQC Report
Sun 4 Sep 2022
EGAF00005002529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences899340
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCTTGACTCAGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG17840.19836769186292172No Hit
GGTCCTTGACTCAGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG16180.1799097115662597No Hit
GTTCCTTGACTCAGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG16080.1787977850423644No Hit
TGTCCTTGACTCAGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG15910.1769075099517424No Hit
GTTCCTTGACGAGGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG15860.17635154668979475No Hit
TATCCTTGATAAGCCCAGGGTTTTCCCAGTCACGACACGATACAACATGA14370.15978384148375477No Hit
ATTCCTTGACTCAGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG13380.14877576889719127No Hit
TTTCCTTGACGAGGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG12810.14243778771098806No Hit
TTTCCTTGATAAGCCCAGGGTTTTCCCAGTCACGACACGATACAACATGA12440.13832365957257545No Hit
GGTCCTTGATGCACCCAGGGTTTTCCCAGTCACGACACGATACAACATGA12410.13799008161540685No Hit
AGTCCTTGACTCAGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG12120.1347654946961105No Hit
GGTCCTTGACAACTCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG11950.13287521960548845No Hit
TATCCTTGACTCAGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG11920.13254164164831989No Hit
TTTCACGGATGTTGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG11840.13165210042920364No Hit
TGTCCTTGATAAGCCCAGGGTTTTCCCAGTCACGACACGATACAACATGA11730.13042898125291882No Hit
GGTCCTTGACGAGGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG11450.12731558698601197No Hit
TATCCTTGAAGGAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA11400.1267596237240643No Hit
GATCCTTGACGAGGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG11370.12642604576689573No Hit
GGTCCTTGATAAGCCCAGGGTTTTCCCAGTCACGACACGATACAACATGA11270.12531411924300043No Hit
TTTCCTTGACTCAGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG11240.12498054128583183No Hit
TTTCCTTGATGTTGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG11210.12464696332866324No Hit
TATCCTTGACGAGGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG11160.1240910000667156No Hit
TGTCCTTGAAGGAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA11050.12286788089043077No Hit
ATTCCTTGACGAGGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG11050.12286788089043077No Hit
ATTCCTTGATGTTGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG10960.121867147018925No Hit
GATCCTTGACTCAGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG10940.12164476171414593No Hit
GTTCCTTGACGAGGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG10900.12119999110458782No Hit
AGTCCTTGACGAGGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG10740.11942090866635532No Hit
TGTCCTTGACTCAGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG10730.1193097160139658No Hit
GTTCCTTGACGAGGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA10700.1189761380567972No Hit
TTTCACGGACTCAGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG10670.11864256009962862No Hit
TGTCCTTGACAACTCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG10610.11797540418529144No Hit
TATCCTTGATGTTGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG10560.11741944092334379No Hit
GGTCCTTGACTCAGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG10490.11664109235661707No Hit
ATTCACGGAAGGAGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG10350.11508439522316366No Hit
TTTCCTTGACTCAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA10350.11508439522316366No Hit
TGTCCTTGACGAGGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG10320.11475081726599505No Hit
GTTCCTTGAGGAATCCAGGGTTTTCCCAGTCACGACACGATACAACATGA10310.11463962461360554No Hit
TTTCACGGAAGGAGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG10310.11463962461360554No Hit
ATTCCTTGAGGAATCCAGGGTTTTCCCAGTCACGACACGATACAACATGA10300.114528431961216No Hit
TCTCCTTGACTCAGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG10240.11386127604687882No Hit
GATCCTTGAAGGAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA10150.11286054217537304No Hit
TTTCACGGATGTTGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA10080.11208219360864635No Hit
GTTCCTTGACTCAGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG10030.11152623034669869No Hit
AATCCTTGAAGGAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA9990.11108145973714056No Hit
ATTCCTTGATGCACCCAGGGTTTTCCCAGTCACGACACGATACAACATGA9930.1104143038228034No Hit
TTTCCTTGAGGAATCCAGGGTTTTCCCAGTCACGACACGATACAACATGA9910.11019191851802433No Hit
AATCCTTGATGTTGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG9870.10974714790846622No Hit
AGTCCTTGATGTTGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG9860.10963595525607668No Hit
GGTCCTTGACAACTCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG9800.1089687993417395No Hit
TGTCCTTGAAGGAGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG9780.10874641403696043No Hit
TGTCACGGATGTTGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG9690.10774568016545466No Hit
CGTCACGGAGGAATCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG9650.10730090955589655No Hit
TATCCTTGAAGGAGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG9640.10718971690350702No Hit
GTTCACGGATGTTGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG9610.10685613894633843No Hit
TATCCTTGATGCACCCAGGGTTTTCCCAGTCACGACACGATACAACATGA9560.10630017568439076No Hit
TGTCCTTGATGTTGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG9550.10618898303200125No Hit
GTTCCTTGATGTTGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG9550.10618898303200125No Hit
CTTCCTTGACTCAGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG9550.10618898303200125No Hit
GGTCCTTGACTCAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA9530.1059665977272222No Hit
GTTCCTTGATGCACCCAGGGTTTTCCCAGTCACGACACGATACAACATGA9510.10574421242244313No Hit
GTTCCTTGATAAGCCCAGGGTTTTCCCAGTCACGACACGATACAACATGA9490.10552182711766407No Hit
ATTCCTTGATAAGCCCAGGGTTTTCCCAGTCACGACACGATACAACATGA9350.10396512998421063No Hit
AGTCCTTGAAGGAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA9350.10396512998421063No Hit
TGTCCTTGACTCAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA9280.10318678141748394No Hit
GGTCCTTGAAGGAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA9210.10240843285075722No Hit
AGTCCTTGATGCACCCAGGGTTTTCCCAGTCACGACACGATACAACATGA9200.10229724019836768No Hit
GTTCCTTGACTCAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA9180.10207485489358863No Hit
GTTCACGGAAGGAGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG9120.10140769897925145No Hit
CGTCACGGACAACTCCAGGGTTTTCCCAGTCACGACACGATACAACATGA9080.10096292836969332No Hit
GATCCTTGAAGGAGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG9080.10096292836969332No Hit
TATCCTTGATGTTGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA9060.10074054306491427No Hit
GATCCTTGATAAGCCCAGGGTTTTCCCAGTCACGACACGATACAACATGA9020.10029577245535615No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)