FastQCFastQC Report
Thu 1 Sep 2022
EGAF00005002539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18012
Sequences flagged as poor quality0
Sequence length251
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG7073.925161003775261No Hit
AGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG6213.4477015323117923No Hit
TGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG6133.4032866977570504No Hit
CGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG5963.308905174328226No Hit
CGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG5062.809238285587386No Hit
AGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG5042.798134576948701No Hit
GGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3571.9820119920053298No Hit
CATATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG3231.7932489451476792No Hit
TGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA2901.6100377526093717No Hit
CTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG2851.5822784810126582No Hit
GGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG2811.5600710637352875No Hit
CGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA2781.5434155007772596No Hit
AATATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG2701.4990006662225184No Hit
AGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA2601.4434821230290917No Hit
CTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG2401.3324450366422385No Hit
CATATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG2401.3324450366422385No Hit
GTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG2091.160337552742616No Hit
GTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG2081.1547856984232734No Hit
AATATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG2051.1381301354652453No Hit
TATATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG1981.0992671552298467No Hit
TATATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG1961.0881634465911614No Hit
ATTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG1961.0881634465911614No Hit
TGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCTAAGATGCCTAA1931.0715078836331335No Hit
TTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG1831.015989340439707No Hit
GATATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG1720.9549189429269377No Hit
TTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG1710.949367088607595No Hit
ATTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG1660.9216078170108817No Hit
CCTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG1630.9049522540528536No Hit
CGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCTAAGATGCCTAA1520.8438818565400843No Hit
CCTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG1500.8327781479013991No Hit
GATATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG1490.8272262935820565No Hit
GGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA1460.8105707306240285No Hit
TGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT1430.7939151676660005No Hit
AGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCTAAGATGCCTAA1420.7883633133466578No Hit
TCTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG1400.7772596047079724No Hit
CATATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA1390.7717077503886298No Hit
TGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT1340.7439484787919165No Hit
TGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCCTGATGGATCAA1340.7439484787919165No Hit
GCTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG1330.7383966244725738No Hit
AGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCCTGATGGATCAA1270.7050854985565178No Hit
AATATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA1240.6884299355984899No Hit
CGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCCTGATGGATCAA1230.6828780812791473No Hit
GTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA1200.6662225183211192No Hit
AGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT1160.6440151010437486No Hit
CTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA1160.6440151010437486No Hit
AGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT1150.638463246724406No Hit
CGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT1150.638463246724406No Hit
TCTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG1150.638463246724406No Hit
TGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG1150.638463246724406No Hit
ACTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG1100.6107039751276927No Hit
GCTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG1030.571840994892294No Hit
CGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT1030.571840994892294No Hit
ACTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG1020.5662891405729514No Hit
ATTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA1000.5551854319342661No Hit
CATATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCTAAGATGCCTAA960.5329780146568954No Hit
GGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCTAAGATGCCTAA840.4663557628247834No Hit
TTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA840.4663557628247834No Hit
CGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG830.46080390850544084No Hit
TATATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA820.45525205418609815No Hit
AGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG780.4330446369087275No Hit
CTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCTAAGATGCCTAA760.42194092827004215No Hit
TCTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA760.42194092827004215No Hit
CCTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA730.40528536531201426No Hit
GGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT720.39973351099267157No Hit
GGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCCTGATGGATCAA710.3941816566733289No Hit
GCTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA670.37197423939595825No Hit
CTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT660.36642238507661556No Hit
GATATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA660.36642238507661556No Hit
TGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC640.35531867643793025No Hit
CGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC640.35531867643793025No Hit
CATATGAGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT620.34421496779924493No Hit
CATATGAGACAACTCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT620.34421496779924493No Hit
CTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCCTGATGGATCAA610.3386631134799023No Hit
GGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT610.3386631134799023No Hit
AATATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCTAAGATGCCTAA610.3386631134799023No Hit
GTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCTAAGATGCCTAA590.327559404841217No Hit
CCTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCTAAGATGCCTAA590.327559404841217No Hit
TTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCTAAGATGCCTAA590.327559404841217No Hit
AGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC580.3220075505218743No Hit
TATATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCTAAGATGCCTAA560.310903841883189No Hit
GTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCCTGATGGATCAA550.30535198756384635No Hit
CATATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCCTGATGGATCAA540.29980013324450366No Hit
CATATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG500.27759271596713303No Hit
CTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT490.27204086164779034No Hit
AATATGAGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT490.27204086164779034No Hit
ATTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCCTGATGGATCAA480.2664890073284477No Hit
ATTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCTAAGATGCCTAA470.2609371530091051No Hit
GATATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCTAAGATGCCTAA440.24428159005107705No Hit
TATATGAGACAACTCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT430.23872973573173442No Hit
GATATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCCTGATGGATCAA430.23872973573173442No Hit
GGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG430.23872973573173442No Hit
TTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCCTGATGGATCAA430.23872973573173442No Hit
AATATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCCTGATGGATCAA420.2331778814123917No Hit
GTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG420.2331778814123917No Hit
ACTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA410.22762602709304908No Hit
TCTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCTAAGATGCCTAA410.22762602709304908No Hit
GCTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCTAAGATGCCTAA400.22207417277370642No Hit
TATATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCCTGATGGATCAA400.22207417277370642No Hit
CCTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT390.21652231845436376No Hit
AATATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG380.21097046413502107No Hit
AATATGAGACAACTCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT360.19986675549633579No Hit
ACTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCTAAGATGCCTAA350.1943149011769931No Hit
GATATGAGACAACTCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT350.1943149011769931No Hit
CTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG350.1943149011769931No Hit
TCTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCCTGATGGATCAA350.1943149011769931No Hit
CCTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCCTGATGGATCAA340.18876304685765047No Hit
GCTATGAGACAACTCCAGGGTTTTCCCAGTCACGACGCCTGATGGATCAA340.18876304685765047No Hit
GGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC340.18876304685765047No Hit
GATATGAGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT320.17765933821896512No Hit
AGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCTCAGCAGAGATGC320.17765933821896512No Hit
CATATGAGACAACTCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC310.17210748389962247No Hit
TTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT300.1665556295802798No Hit
TTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG290.16100377526093715No Hit
TATATGAGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT290.16100377526093715No Hit
GTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT280.1554519209415945No Hit
AATATGAGACAACTCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC280.1554519209415945No Hit
GTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT280.1554519209415945No Hit
ATTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT280.1554519209415945No Hit
TATATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG270.14990006662225183No Hit
ATTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT270.14990006662225183No Hit
TTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT270.14990006662225183No Hit
TGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCTCAGCAGAGATGC270.14990006662225183No Hit
TCTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT250.13879635798356652No Hit
GATATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG250.13879635798356652No Hit
ATTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG230.12769264934488117No Hit
CCTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT230.12769264934488117No Hit
TCTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT230.12769264934488117No Hit
ACTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT230.12769264934488117No Hit
GTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC230.12769264934488117No Hit
CGTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCTCAGCAGAGATGC220.12214079502553853No Hit
GCTATGAGACAACTCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG220.12214079502553853No Hit
ATTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC220.12214079502553853No Hit
GATATGAGACAACTCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC210.11658894070619585No Hit
GCTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT210.11658894070619585No Hit
CTTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC210.11658894070619585No Hit
GCTATGAGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT210.11658894070619585No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)