FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490464946
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG8066130.16445884799277785TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2151900.070.262451
TCGGAAG2315950.064.981122
CGGAAGA2531000.059.3958743
AGAGCAC5403350.028.2219437
AGCACAC5832450.026.2152239
GAAGAGC5951200.025.84075
GAGCACA5928450.025.8151268
TATGCCG1707700.018.41825145-49
AAGAGCA8696650.017.8905986
CTCGTAT1615850.017.04867640-44
CGTATGC1846500.016.94189545-49
TCTCGTA1659050.016.60562140-44
GGAAGAG9683000.016.2085484
CGCTCAT1944100.016.14682830-34
ATGCCGT1956200.015.97922545-49
TATCTCG1717500.015.85815140-44
GCCGTCT1997500.015.37691650-54
ACCGCTC2057650.015.3177830-34
TGCCGTC1966200.015.14284345-49
GTCACCG2153300.014.84909925-29
Produced by FastQC (version 0.11.5)