FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490464946
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC9492430.19353941759580917Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2888950.051.9597559
GAGCGTC3223100.046.66488
ATCGGAA3478450.044.101961
AGAGCGT3615800.041.6910557
TCGGAAG3690750.041.3977742
CGGAAGA3958000.038.462183
AAGAGCG4261300.035.766976
GAAGAGC7357700.021.1620925
CGCCGTA1993950.015.52782155-59
TCGCCGT2099750.014.73575455-59
GCCGTAT2204250.014.15620355-59
GGAAGAG11376150.014.0781994
GTCGCCG2344350.013.47224555-59
TGGTCGC2438450.013.00657650-54
CGTATCA2405550.012.93826460-64
CCGTATC2305100.012.90853955-59
GTGGTCG2613300.012.22731950-54
GGTCGCC2567700.011.90467750-54
GCGTCGT2786300.011.2858110-14
CGTCGTG2819150.011.19544110-14
Produced by FastQC (version 0.11.5)