FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463864021
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC12001660.2587322891335002Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3340600.056.703769
GAGCGTC3735000.050.7692958
ATCGGAA3921950.049.0687141
AGAGCGT4018350.047.231447
TCGGAAG4089550.047.0201072
CGGAAGA4344200.044.0887953
AAGAGCG4655700.041.147786
GAAGAGC7587700.025.6301175
GGAAGAG11391450.017.3762824
CGCCGTA2394850.016.11521155-59
TCGCCGT2499950.015.43655355-59
GCCGTAT2558100.015.13437555-59
GTCGCCG2779850.014.100697555-59
CCGTATC2687400.014.06378255-59
CGTATCA2760750.013.95431960-64
TGGTCGC2851800.013.85781950-54
GTGGTCG3015300.013.1679250-54
GGTCGCC2981950.013.02066950-54
TCCGATC376200.012.7679343
TCTTCGC3341500.011.84583430-34
Produced by FastQC (version 0.11.5)