FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473348278
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC8681820.1834129414536499Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3381850.040.2654239
GAGCGTC3717400.036.7303168
ATCGGAA4146900.033.5166361
AGAGCGT4218150.032.5503657
TCGGAAG4293600.032.367332
CGGAAGA4554900.030.529583
AAGAGCG4868200.028.5047136
GAAGAGC7859050.018.0386315
CGCCGTA2293500.012.25790455-59
GGAAGAG11998000.012.1450864
TCCGATC360750.012.0428723
TCGCCGT2380700.011.78273955-59
GCCGTAT2527050.011.23746655-59
GTCGCCG2546750.011.13118755-59
CCGTATC2604350.010.57328555-59
TGGTCGC2727150.010.49420750-54
CGTATCA2727950.010.38872260-64
GGTCGCC2753450.010.13283150-54
GTGGTCG2951450.09.77330750-54
GCGTCGT3209150.08.71280110-14
Produced by FastQC (version 0.11.5)