FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324823727
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG3374820.10389696686166033TruSeq Adapter, Index 13 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1203350.054.4820141
TCGGAAG1306300.050.0992052
CGGAAGA1484550.044.078123
AGAGCAC3395850.019.859857
AGCACAC3656050.018.438339
GAGCACA3705100.018.2940448
GAAGAGC3739850.018.0347295
TATGCCG896200.014.98231945-49
CTCGTAT819900.013.56484340-44
CGTATGC998150.013.43606345-49
TCTCGTA844050.013.06335440-44
AAGAGCA5541700.012.4790826
ATGCCGT1090300.012.3815945-49
TGCCGTC1072950.012.22776845-49
GCCGTCT1115750.011.85087250-54
TCGTATG976050.011.20755440-44
GGAAGAG6233100.011.1797844
ATCTCGT1007950.011.08875340-44
CGTCTGA1295800.010.52412515-19
CGTCTTC1304700.010.44352650-54
Produced by FastQC (version 0.11.5)