FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386638959
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG26101330.6750827714700112TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA4309200.0108.439961
TCGGAAG4462300.0104.5701452
CGGAAGA4677700.099.54253
AGAGCAC6914500.067.417717
AGCACAC7228300.064.4754949
GAAGAGC7333750.063.714275
GAGCACA7327950.063.5534448
AAGAGCA9503650.049.2575576
GGAAGAG10394250.045.218824
TATGCCG3868900.024.55839545-49
CTCGTAT3512650.023.96417240-44
TCTCGTA3533350.023.7991640-44
TCGTATC4010150.023.70113235-39
CGTATGC3989200.023.5459345-49
ATGCCGT4037550.023.26971245-49
TATCTCG3579650.023.1583940-44
GTCACGA4202950.022.74351125-29
GCCGTCT4061650.022.70763250-54
ATCTCGT3722800.022.5440340-44
TGCCGTC4042700.022.53674745-49
Produced by FastQC (version 0.11.5)