FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386638959
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC28937250.7484307860450244Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG5265050.087.918079
GAGCGTC5538550.083.6123058
ATCGGAA5820550.080.860561
TCGGAAG5979900.078.5542452
AGAGCGT5925100.078.278347
CGGAAGA6197500.075.701363
AAGAGCG6489600.072.079686
GAAGAGC8886450.052.95085
GGAAGAG12280250.038.6222344
CGCCGTA4250550.021.37193555-59
TCGCCGT4301200.020.95038855-59
GCCGTAT4387300.020.7183455-59
GTCGCCG4714550.019.922655-59
CGTATCA4525550.019.85601260-64
TGGTCGC4754550.019.846350-54
CCGTATC4457950.019.80300155-59
GTGGTCG4961600.019.09290750-54
GGTCGCC4901000.018.88971350-54
GCGTCGT5076350.018.45397610-14
CGTCGTG5105450.018.37207810-14
Produced by FastQC (version 0.11.5)